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Quantitative analysis of chimerism after allogenichematopoietic cells transplantation


Authors: J. Dušková;  Z. Broukal
Authors‘ workplace: Centrum molekulární biologie a genové terapie, Interní hematoonkologická klinika, FN Brno
Published in: Prakt. Lék. 2004; (10): 70-75
Category:

Overview

Examination of chimerism has become an integral element of the monitoring of patients after allogenichematopoietic cell transplantation. Presented publication initially describes different methods ofchimerism evaluation. In the second part, there is detailed characterization of the capillary electrophoresiswith fluorescence detection (fragment analysis) of amplification products of high polymorficmicro- and mini- satellite DNA regions, technique, which become the gold standard for quantitativeevaluation.

Key words:
chimerism, STR and VNTR polymorphism, fragment analysis

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