Authors: M. Havlíčková; A. Štumpa; I. Holíková; Z. Horníčková Authors‘ workplace: Národní referenční laboratoř pro chřipku a NRLpro nechřipkové respirační viry, Státní zdravotní ústav, Praha, ředitel doc. MUDr. J. Kříž Published in: Prakt. Lék. 1999; (5): 272-274 Category:
The authors elaborated an ELISA system enabling specific detection of IgM antibodies againsttype A influenza and RSV. The aim of the study was to verify the practicability of this methodfor rapid diagnosis, particularly in those cases where it was not possible to take a nasopharyn-geal smear during the acute phase of the disease.Detection of RSV antibodies was tested in a cohort of 25 patients where the aetiology wasdemonstrated by isolation of viruses and a marked decline of IgG antibody titres. IgM antibodiesin the first or second serum samples were detected in 23 cases (92 %). The 34 negative pairedsera, serving as controls, gave negative results only. IgM antibodies against type A influenzawere tested on a set of 40 paired sera with confirmed seroconversion; positive IgM titres weredetected in a convalescent serum samples only in 35 examined cases (87.7 %). Of 15 controlnegative paired sera, none had a positive IgM titre.Our results show that IgM antibodies against RSV and type A influenza virus appear relativelylate following onset of disease and last for 4-10 weeks in the case of RSV and up to 3 monthsfor influenza. Therefore, detection of IgM cannot be accepted as proof of acute infection withthese viruses.
Key words: IgM antibodies - RSV - influenza - diagnosis.
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