Diagnosis of Early Phase Diffuse Axonal Injury by Immunohistochemical Methods

Overview

Histologic and immunohistochemical investigation of the brain aimed at diagnosis of diffuse axonal injury (DAI) was performed in a group of 12 persons deceased of craniocerebral injury. Traumatic axonal change was visualized by immunohistochemical positivity of ubiquitin, low-mo- lecular neurofilaments, beta-amyloid precursor protein and neuron-specific enolase. In addition, H-E stain and silver impregnation of axons according to Palmgren were performed. Diffuse axonal injury was found in 7 cases (58 %). In persons surviving more than 12 hours, morphological diagnossis of DAI was based on the finding of so called retraction balls. In person deceased in the first hours after injury, the retraction balls had not been formed yet and DAI was diagnosed according to histochemistry of axonal lesion. The earliest diagnosis of DAI was estab- listed 2 hours after injury when axonal lesion showed an immunohistochemical positivity with anti-neuron- specific enolase. In a control group of 6 sudden death cases, axonal oedema was also found but lacking immunohis- tochemical positivity. Immunohistochemical proof, specially of neuron-specific enolase, seems to be inevitable for dia- gnosis of early phase DAI when the traumatic etiology of axonal deformities is to be distinguished from possible artificial lesions.

Key words:
diffuse axonal injury - immunohistochemistry - beta-amyloid perkursor protein - neuron-specific-enolase - low-molecular neurofilament - ubiquitin