Profi ly endometriální exprese mRNAu vybraných matrix metaloproteináza tkáňového inhibitoru metaloproteináz-1

Overview

Objective:
To examine the endometrial expression pattern of messenger RNA (mRNA) for selectedmatrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1).Design: Experimental study.Setting: Department of Obstetrics and gynecology of the Palacky University Medical School andUniversity Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, UniversityHospital, Luna, Sweden.Methods: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normalendometrial samples obtained across the menstrual cycle. Based on histological examination, allspecimens were classifi ed according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6)and late (n=7) proliferative phase, early (n=4), mid (n=4) and late (n=8) secretory phase and menstrual(n=3) phase. mRNA extracted from frozen tissue samples was quantitated using real timePCR.Results: Four distinct patterns of MMP mRNA expression were detected in cycling endometrium.MMP-1, -3, -10, and -12 were expressed predominantly in perimenstrual period, MMP-7 and -11had highest levels in proliferative phase, MMP-13, -14 and -16 were expressed throughout thecycle and MMP-26 was found to be maximal in periovulatory period. Levels of TIMP-1 mRNAremained unchanged during the cycle.Conclusion: The specifi c endometrial expression profi les of MMPs during menstrual cycle pointto their specifi c biologic roles during the cycle. MMP-26 exhibits a unique expression pattern.

Klíčová slova:
endometrium, MMP, mRNA, real time PCR, TIMP