Authors: Vanda Repiská 1; J. Vojtaššák 1; M. Korbeľ 2; Ľudovít Danihel 3; Šu&# 64258; J. Iarsky 4; Z. Nižňanská 2; M. Redecha 2; I. Ilavská 4 Authors‘ workplace: Ústav lekárskej biológie a genetiky LF UK v Bratislave, prednosta doc. RNDr. J. Vojtaššák, CSc. 21. gynekologicko-pôrodnícka klinika LF UK a FN v Bratislave, prednosta prof. MUDr. M. Borovský, CSc. 3Ústav patologickej anatómie LF UK a FN v Bratislave, pre 1 Published in: Ceska Gynekol 2003; (6): 442-448 Category:
Objective: DNA analysis of different forms of gestational trophoblastic disease.Design: Retrospective clinical study.Setting: Slovak Center of Trophoblastic Disease, Bratislava, Slovak Republic.Methods: In the period of September 1993 to April 2003, eighty-nine cases of gestational trophoblasticdisease were analysed. There were 22 cases of partial hydatidiform moles, 58 cases of completehydatidiform mole, 5 cases of invasive mole and 4 cases of gestational choriocarcinomas.Southern hybridization and polymerase chain reaction were used for DNA analysis.Results: From 22 analyzed cases of partial hydatidiform moles 19 (86.4%) were triploid and 3(13.6%) diploid ones. There were 58 cases of complete hydatidiform mole and out of them 29 (50%) were homozygous, 28 (48.3%) heterozygous, and in one case (1.7%) both paternal and maternalgenome was detected. In 8 cases of heterozygous and in one case of homozygous complete hydatidiformmole occurred a malignant transformation to gestational choriocarcinoma.Conclusions: Molecular analysis can determine the nuclear DNA origin of complete hydatidiformmole and allow us to defi ne the patients with higher risk of malignant transformation usually togestational choriocarcinoma.
Key words: DNA analysis, gestational trophoblastic disease, hydatidiform mole, choriocarcinoma
Don‘t have an account? Create new account
Enter the email address that you registered with. We will send you instructions on how to set a new password.
