Effect of abiotic elicitation on the sanguinarine production and polyphenol oxidase activity in the suspension culture of Eschscholtzia californica CHAM.
Authors:
František Bilka; Andrea Balážová; Andrea Bilková; Ivana Holková
Authors‘ workplace:
Katedra bunkovej a molekulárnej biológie liečiv
; Univerzita Komenského v Bratislave, Farmaceutická fakulta
Published in:
Čes. slov. Farm., 2013; 62, 169-173
Category:
Original Articles
Overview
Elicitation of plant in vitro cultures represents a biotechnological tool to improve the production of secondary metabolites. In this study, the effect of AgNO3 and CdCl2 on the sanguinarine production by the suspension culture of Eschscholtzia californica CHAM. was investigated. Elicitors were added to the cultures at the 14th day of subcultivation and their effect on the sanguinarine production was evaluated after a 48 h exposure. AgNO3 at the concentration of 0.075 mmol.l-1 and CdCl2 at the concentration of 4 mmol.l-1 induced a ca. 5.2- and 5.6-multiple increase in sanguinarine synthesis, respectively. This amount represents probably the maximal production, because a further increase in the elicitors concentrations did not increase sanguinarine production. Both abiotic elicitors induced a polyphenol oxidase specific activity increase. Polyphenol oxidase is probably involved in the biosynthesis of sanguinarine at the level of dopamine formation. Dopamine is a precursor of (S)-norcoclaurine, the first intermediate with the benzylisoquinoline structure.
Keywords:
Eschscholtzia californica CHAM. • suspension cultures • abiotic elicitation • sanguinarine • polyphenol oxidase
Sources
1. Zdařilová A., Malíková J., Dvořák Z., Ulrichová J., Šimánek V. Kvartérní isochinolinové alkaloidy sanguinarin a chelerythrin. Účinky in vitro a in vivo. Chem. Listy 2006; 100, 30–41.
2. Bilková A., Bilka F., Kiňová Sepová H., Balažová A. Testovanie potenciálne probiotických laktobacilov pre použitie vo výživových doplnkoch. Čes. slov. Farm. 2013; 62, 40–45.
3. Hakim S. A. E., Mijovič V., Walker J. Experimental transmission of sanguinarine in milk detection of a metabolic product. Nature 1961; 189, 201.
4. Dvořák Z., Šimánek V. Metabolism of sanguinarine: The facts and the myths. Current Drug Metabolism 2007; 8, 173–176.
5. Bilka F., Balažová A., Bilková A., Holková I. Porovnanie produkcie sanguinarínu suspenznými kultúrami rastlín čeľade Papaveraceae. Čes. slov. Farm. 2012; 61, 267–270.
6. Sikyta B., Dušek, J. Biotechnologie pro farmaceuty. 3. vyd. Praha: Univerzita Karlova – Nakladatelství Karolinum 2001; 125 s.
7. Collin H. A. Secondary product formation in plant tissue cultures. Plant Growth Reg. 2001; 34, 119–134.
8. Verpoorte R., Contin A., Memelink J. Biotecnology for the production of plant secondary metabolites. Phytochemistry Rev. 2002; 1, 13–25.
9. Radman R., Saez T., Bucke C., Keshavarz T. Elicitation of plants and microbial cell systems. Biotechnol. Appl. Biochem. 2003; 37, 91–102.
10. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 1962; 15, 473–497.
11. Bilková A., Bilka F., Blanáriková V., Bezáková L. Effect of excess of cupric sulfate on sanguinarine formation and activities of amine oxidase and polyphenol oxidase in cell suspension cultures of Papaver somniferum. Biologia 2005; 60, 661–664.
12. Balažová A., Bilka F., Blanáriková V., Pšenák M. Zmeny obsahu sanguinarínu a aktivity polyfenoloxidázy vplyvom fungálneho elicitora v suspenzných kultúrach maku siateho Papaver somniferum L. Čes. Slov. Farm. 2002; 51, 182–185.
13. Escribano J., Cabanes J., Chazarra S., Garcıa-Carmona F. Characterization of monophenolase activity of table beet polyphenoloxidase. Determination of kinetic parameters on the tyramine/dopamine pair. J. Agric. Food Chem. 1997; 45, 4209–4214.
14. Bilka F., Vanko M., Balažová A., Bilková A., Holková I.: Charakterizácia polyfenoloxidázy z latexu lastovičníka väčšieho (Chelidonium majus L.). Čes. Slov. Farm. 2007; 56, 90–94.
15. Lineweaver H., Burk D. The determination of enzyme dissociation constants. J. Amer. Chem. Soc. 1934; 56, 658–666.
16. Bradford M. M. A rapid and sensitive method for the quantication of microgram quantites of protein utiliting the principle of protein-dye binding. Anal. Biochem. 1976; 72, 248.
17. Tůmová L., Polívková D. Vliv AgNO3 na produkci flavonoidů kulturou Ononis arvensis L. in vitro. Čes. slov. Farm. 2006; 55, 186–188.
18. Siatka T., Kašparová M., Spilková J. Effects of zinc and cadmium ions on cell growth and production of coumarins in cell suspension cultures of Angelica archangelica L. Čes. slov. Farm. 2012; 61, 261–266.
19. Villegas M., Sommarin M., Brodelius P. E. Effects of sodium orthovanadate on benzophenanthridine alkaloid formation and distribution in cell suspension cultures of Eschscholtzia californica. Plant Physiol. Biochem. 2000; 38, 233–241.
20. Balažová A., Blanáriková V., Bilka F., Bilková A., Kiňová Sepová, H. Efekt troch rôznych elicitorov na produkciu sanguinarínu suspenznými kultúrami nízko-morfínovej odrody maku siateho (Papaver somniferum L.). Čes. slov. Farm. 2011; 60, 237–240.
21. Hwa-Young Ch., Rhee H. S., Yoon S. Y. H., Moon Park J. Differential induction of protein expression and benzophenanthridine alkaloid accumulation in Eschscholtzia californica suspension cultures by methyl jasmonate and yeast extract. J. Microbiol. Biotechnol. 2008; 18, 255–262.
22. Wahler D., Gronover Ch. S., Richter C., Foucu F., Twyman R. M., Moerschbacher B. M., Fischer R., Muth J., Prűfer D. Polyphenoloxidase silencing affects latex coagulation in Taraxacum species. Plant Physiol. 2009; 151, 334–346.
23. Bilková A., Bilka F., Bezáková L. Enzymológia tvorby benzylizochinolínových alkaloidov. Čes. slov. Farm. 2005; 54, 17–22.
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Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
2013 Issue 4
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