Administration of 500 ml of normal saline does not result in immediate damage to the endothelial glycocalyx determinable by sublingual microcirculation assessment
Authors:
J. Pouska 1,2; D. Astapenko 3,4; V. Tégl 1,2,5; J. Beneš 1,2,5; V. Černý 3,4,6-9
Authors‘ workplace:
Klinika anesteziologie, resuscitace a intenzivní medicíny, Fakultní nemocnice Plzeň
1; Klinika anesteziologie, resuscitace a intenzivní medicíny, Lékařská fakulta v Plzni, Univerzita Karlova
2; Klinika anesteziologie, resuscitace a intenzivní medicíny, Fakultní nemocnice Hradec Králové
3; Lékařská fakulta v Hradci Králové, Univerzita Karlova
4; Biomedicínské centrum, Lékařská fakulta v Plzni, Univerzita Karlova
5; Klinika anesteziologie, perioperační a intenzivní medicíny, Univerzita J. E. Purkyně, Masarykova nemocnice, Ústí nad Labem
6; Institut postgraduálního vzdělávání ve zdravotnictví
7; Centrum pro výzkum a vývoj, Fakultní nemocnice Hradec Králové
8; Dept. of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, Kanada
9
Published in:
Anest. intenziv. Med., 29, 2018, č. 6, s. 322-327
Category:
Overview
Objective:
Assessment of endothelial glycocalyx alteration after infusion of 500 ml of normal saline by perfused boundary region (PBR) parameter in twelve healthy volunteers.
Hypothesis:
Endothelial glycocalyx will be significantly altered by infusion of 500 ml of normal saline.
Design:
Non-randomized, prospective, double-centre study.
Settings:
Two ICUs in University Hospitals.
Materials and methods:
Twelve healthy volunteers without acute illness were recruited. 500 ml of normal saline was infused to each participant. We collected basic haemodynamic data as well as perfused boundary region as a marker of endothelial glycocalyx integrity before and after the fluid administration. Student T-test (paired) with p-value of 0.05 was used for the statistical analysis.
Results:
After administration of 500 ml of normal saline there was non-significant change in PBR (2.00±0.21 vs. 2.21±0.36, p = 0.089). Concerning the macrohaemodynamics, there was a significant drop in the heart rate after the infusion (74±10 vs. 68±10, p = 0.003).
Conclusion:
Our study did not confirm alteration of the endothelial glycocalyx after intravenous administration of 500 ml of normal saline to healthy volunteers. There was a significant decrease of heart rate after the infusion but no other changes were observed.
keywords
microcirculation – intravenous infusion – endothelial glycocalyx
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Anaesthesiology, Resuscitation and Inten Intensive Care MedicineArticle was published in
Anaesthesiology and Intensive Care Medicine
2018 Issue 6
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