Stability of coagulation factors in thawed plasma
Authors:
M. Bohoněk 1; V. Mašková 1; E. Sládková 1; I. Hrachovinová 2; M. Petráš 3
Authors‘ workplace:
Oddělení hematologie a krevní transfuze, Ústřední vojenská nemocnice – Vojenská fakultní nemocnice Praha
1; Laboratoř pro poruchy hemostázy, Ústav hematologie a krevní transfuze Praha
2; 2. lékařská fakulta Univerzity Karlovy v Praze
3
Published in:
Transfuze Hematol. dnes,19, 2013, No. 4, p. 229-239.
Category:
Comprehensive Reports, Original Papers, Case Reports
Overview
Massive blood transfusion protocol requires having lots of blood products handy as soon as possible. Thawing of FFP costs time, therefore it is desirable to have stock of thawed plasma in hospital emergency room. The aim of study is evaluation of quality of fresh frozen plasma after thawing and during following 5day storage at 2–6°C for use in handy storage. 10 units of FFP various blood groups stored at -25°C were randomly selected and thawed. Samples were kept at 2–6°C for 5 days and tested for various coagulation factors each day during storage. Measured values were analysed using the Wilcoxon matched-pairs test and Friedman Test (nonparametric repeated measures ANOVA). All tests were two-tailed with the level of significance set at 0.05. At most of studied parameters were detected gradual decreases of levels. At Protein S and F V, there was approx. 10% decrease from 3rd day, at serine proteases (F II, F VII and F X) there was approx. 20% decrease up until 4th day. Significant activity decrease of 42.6% from 2nd day was detected at F VIII; in following days, activity was stable. All parameters in thawed FFP stored at 2–6°C for 5 days display sufficient activity for clinical use, but it is preferable that they are used within first 24 hours.
Key words:
fresh frozen plasma, liquid plasma, massive transfusion
Sources
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Labels
Haematology Internal medicine Clinical oncologyArticle was published in
Transfusion and Haematology Today
2013 Issue 4
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