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Influence of processing of platelet lysate at selected of platelets factors


Authors: B. Kubešová
Authors‘ workplace: Tkáňové zařízení ;  Kryo s. r. o.
Published in: Transfuze Hematol. dnes,19, 2013, No. 4, p. 223-228.
Category: Comprehensive Reports, Original Papers, Case Reports

Overview

The author focused on factors from platelet alpha granules, specifically the PDGF, IGF-1, a VEGF and TGF. Platelets were first broken by deep frozen that had washed factors into the plasma. Plasma was heat-treated, thereby leading to precipitation of coagulation factors, as well as to inactivate the complement. After centrifugation platelet lysate was pushed to the bag. Riboflavin solution was added and platelet lysate was viral and bacterial inactivated by Mirasol system. The final solution was freeze-dried.

The author of validated each critical step. Platelet factors were measured at the beginning of the process, following the inactivation of microbial and after freeze-dried. The production process had not a significant impact on the level of platelet factors. This finding is crucial for the other using of platelet lysate.

Key words:
platelet factor, growth factor, platelet lysate, platelet rich plasma


Sources

1. Awidi A, Abu Harfeil N, Jafar H, et al. The in vitro effect of autologous platelet lysate on different types of mesenchymal stem cells. Regenerative Research 2012; 1; 25-32.

2. Schmitz JP, Hollinger JO. The biology of platelet-rich plasma (letter to the editor). J Oral Maxillofac Surg 2001; 59: 1119.

3. Carlson NE, Roach RB Jr. Platelet-rich plasma, clinical applications in dentistry. J Am Dent Association 2002; 133(10): 1383-6.

4. Schliephake H. Bone growth factors in maxillofacial reconstruction. Int J Oral Maxillofacial Surg 2002; 31(5): 469-84.

5. Pietramaggiori G, Kaipainen A, Czeczugy JM, et al. Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds. Wound Repair and Regeneration 2006; 573-580.

6. Marx RE. Platelet-Rich Plasma: Evidence to Support Its Use. J Oral Maxillofac Surg 62 2004; 489-496.

7. Hom DB, Linzie BM, Huang TC. The Healing Effects of Autologous Platelet Gel on Acute Human Skin Wounds. Arch Facial Plast Surg 2007; 174-183.

8. Alio JL, Abad M, Artola A, et al. Use of Autologous Platelet-Rich Plasma in the Treatment of Dormant Corneal Ulcers. American Academy of Opthalmology Published 2007; 1286-1293.

9. Marx RE,Carlson ER,Eistachedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol 1998; 85: 638-646.

10. Bates DO, Lodwick D, Williams B. Vascular endothelial growth factor and micro vascular permeability. Microcirculation 1999; 6(2): 83-96.

11. Yancopoulos GD, et al. Vascular-specific growth factor and blood vessel formation. Nature 2000; 407(6801): 242-248.

12. Bowen-Pope DF, Vogel A, Ross R. Production of platelet derived growth factor receptors accompany transformations by a wide spectrum of agents. Proc Natl Acaid Sci USA 1984; 81: 2396-2400.

13. Singh JP, Chaikin MA, Stiles CD. Phylogenetic analysis of platelet derived growth factor by radio-receptor assay. J Cell Biol 1982; 95: 667-671.

14. Mochizuki H, et al. Insulin-like growth factor-I supports formation and activation of osteoclasts. Endocrinology 1992; 131(3): 1075:1080.

15. Guicheux J, et al. Growth hormone stimulatory effects on osteoclastic resorption are partly mediated by insulin-like growth factor I: an vitro study. Bone 1988; 22(1): 25-31.

16. Cicha I, Garlichs ChD, Daniel WG, et al, Platelets and Blood Cells: Activated human platelets release connective tissue growth factor. Schattauer GmbH, Stuttgard 2004; 755-60.

17. Mirabet V, Solves P, Miňana D, et al. Human Platelet lysate enhances the proliferative activity of cultured human fibroblast-like cells from different tissue. Cell Tissue Banking 2008; 9: 1-10.

18. Kurtz CA, et al. Insulin-like growth factor I accelerates functional recovery from Achilles tendon injury in a rat model. Am J Sports Med 1999; 27(3): 363-369.

19. Aspenberg P, Virchenko O. Platelet concentrate injection improves Achilles tendon repair in rats; Acta Orthopscand 2004; 75 (1): 93-99G.

20. Caloprisco G, Borean A. Chronic skin ulcers: A regenerative simulation by topical hemotherapy; The International Journal of Artificial Organs 2004; 27: 816-817.

21. Fassmann A, Izakovičová-Hollá L, Slapnička J. Kostní tkáňové inženýrství v orofaciální oblasti. Vyd. 1. Hradec Králové: Nucleus HK, 2006; 105 s. edice zubního lékařství.

22. Man D, Plosker H, Winland-Brown J E. Experimental Cosmetic: The Use of Autologous Platelet-Rich Plasma (Platelet Gel) and Autologous Platelet-Poor Plasma (Fibrin Glue) in Cosmetic Surgery. Plastic and Reconstructive Surgery 2001; 229-237.

23. Noble BA, Loh RS, Maclennan S, Pesudovs K, et al. Comparison of autologous serum eye drops with conventional therapy in a randomized controlled crossover trial for ocular surface disease. Br J Opthalmology 2004; 1-9.

Labels
Haematology Internal medicine Clinical oncology
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