Authors: R. Musilová 1; E. Táborská 2; L. Bourková 1; T. Büchler 1,3; L. Hanák 1; L. Kovářová 1,3; H. Novotná 4; M. Penka 1; R. Hájek 1,3 Authors‘ workplace: Laboratoř experimentální hematologie a buněčné imunoterapie, Oddělení klinické hematologie, FN Brno, 2Lékařská fakulta MU, Katedra lékařské chemie a biochemie, 3Interní hematoonkologická klinika, FN Brno, 4Oddělení klinické biochemie, FN Brno 1 Published in: Transfuze Hematol. dnes,, 2002, No. 3, p. 0. Category:
Idiotype vaccination can be used as immunotherapy for B-cell malignancies, including multiplemyeloma. Malignant plasma cells produce monoclonal immunoglobulin that can be detected in theserum and/or urine and is tumour-specific. To isolate the myeloma Id protein we used affinity chromatographyon a protein G column. Id was eluted with 0.2M glycine-HCl. The protein was more than 90 %pure as determined by SDS-polyacrylamide gel electrophoresis. Id protein was conjugated with KLHin the presence of 0.1% glutaraldehyde. The conjugation product was administered in the preclinicalexperiment to test vaccination as a part of the clinical protocol under preparation.
Key words: monoclonal immunoglobulin, idiotypic protein, affinity chromatography
Don‘t have an account? Create new account
Enter the email address that you registered with. We will send you instructions on how to set a new password.
