Changes of plasma obestatin and ghrelin levels after soluble fiber with glucose and after fiber alone in healthy women and in patients with bulimia nervosa
Authors:
Hana Doubková 1; Jana Kopečková 1; Dana Sedláčková 1; Martin Haluzík 3; Hana Kvasničková 1; Hana Papežová 2; Vojtěch Hainer 1; Jara Nedvídková 1
Authors‘ workplace:
Endokrinologický ústav Praha, Laboratoř klinické a experimentální neuroendokrinologie
1; Univerzita Karlova Praha, 1. lékařská fakulta, Psychiatrická klinika VFN
2; Univerzita Karlova Praha, 1. lékařská fakulta, III. interní klinika VFN
3
Published in:
Čas. Lék. čes. 2010; 149: 542-545
Category:
Original Article
Overview
Background.
The recent identification of obestatin, a novel peptide hormone derived from the same gene as ghrelin, has added further complexity to ghrelin physiology. Despite the rapid progress, many questions remain unanswered, including the regulation of orexigen ghrelin and putative anorexigen obestatin secretion by food composition in humans. The present study was undertaken to investigate the influence of caloric and noncaloric food on plasma ghrelin and obestatin concentrations in healthy women (n = 6; age 23.83 ± 1.1 years; BMI 20.85 ± 0.87 kg/m2) and in bulimia nervosa patients (n = 6; age 26.6 ± 5.2 years; BMI 19.2 ± 1.44 kg/m2), characterized by abnormal eating behaviour and imbalance in energy homeostasis.
Methods and Results.
After overnight fasting, plasma ghrelin and obestatin were measured by commercial radioimmunoassay kits before and after consumption of soluble fiber alone or with glucose. In both groups plasma ghrelin and obestatin levels did not change after fiber alone, but decreased after fiber with glucose. During 30–90 min after ingestion we observed significant decrease (p < 0.05) of plasma ghrelin and obestatin levels after soluble fiber with glucose in healthy women and also in patients with bulimia nervosa, after then the levels of both hormones started to increase to preprandial levels.
Conclusions.
We conclude that postprandial ghrelin and obestatin plasma levels decrease in relation to caloric content of the meal in healthy women and in patients with bulimia nervosa.
Key words:
bulimia nervosa, ghrelin, obestatin, fiber.
Sources
1. Nedvídková J, Krykorková I, Barták V, Papežová H, Gold PW, Alesci S, Pacak K. Loss of meal-induced decrease in plasma ghrelin levels in patients with anorexia nervosa. J Clin Endocrinol Metab 2003; 88: 1678–1682.
2. Sedláčková D, Dostálová I, Hainer V, Beranová L, Kvasničková H, Hill M, Haluzik M, Nedvídková J. Simultaneous Decrease of Plasma Obestatin and Ghrelin Levels after a High-Carbohydrate Breakfast in Healthy Women. Physiol Res 2008; 57(Suppl 1): S29.
3. Gasco V, Beccuti G, Marotta F, Benso A, Granata R, Broglio F, Ghigo E. Endocrine and Metabolic Actions of Ghrelin. Endocr Dev 2010; 17: 86–95.
4. Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin’s effects on food intake. Science 2005; 310: 996–999.
5. Lagaud GJ, Young A, Acena A, Morton MF, Barrett TD, Shankley NP. Obestatin reduces food intake and suppresses body weight gain in rodents. Biochem Biophys Res Commun 2007; 357: 264–269.
6. Germain N, Galusca B, Grouselle D, Frere D, Tolle V, Zizzari P, Lang F, Epelbaum J, Estour B. Ghrelin/obestatin ratio in two populations with low bodyweight: Constitutional thinness and anorexia nervosa. Psychoneuroendocrinology 2009; 34: 413–419.
7. Gourcerol G, Coskun T, Craft LS, Mayer JP, Heiman ML, Wang L, Million M, St-Pierre DH, Taché Y. Preproghrelin-derived peptide, obestatin, fails to influence food intake in lean or obese rodents. Obesity 2007; 15: 2643–2652.
8. Monteleone P, Serritella C, Martiadis V, Scognamiglio P, Maj M. Plasma obestatin, ghrelin, and Ghrelin/obestatin ratio are increased in underweight patients with anorexia nervosa but not in symptomatic patients with bulimia nervosa. J Clin Endocrinol Metab 2008; 93: 4418–4421.
9. Nakahara T, Harada T, Yasuhara D, Shimada N, Amitani H, Sakoguchi T, Kamiji MM, Asakawa A, Inui A. Plasma obestatin concentrations are negatively correlated with body mass index, insulin resistance index, and plasma leptin concentrations in obesity and anorexia nervosa. Biol Psychiatry 2008; 64: 252–255.
10. Zamrazilová H, Hainer V, Sedláčková D, Papežová H, Kunešová M, Bellisle F, Hill M, Nedvídková J. Plasma obestatin levels in normal weight, obese and anorectic women. Physiol Res 2008; 57(Suppl 1): S49–S55.
11. Vitiello B, Lederhendler I. Research on eating disorders: current status and future prospects. Biol Psychiatry 2000; 47: 777–786.
12. Tanaka M, Tatebe Y, Nakahara T, Yasuhara D, Sagiyama K, Muranaga T, Ueno H, Nakazato M, Nozoe S, Naruo T. Eating pattern and the effect of oral glucose on ghrelin and insulin secretion in patients with anorexia nervosa. Clin Endocrinol 2003; 59: 574–579.
13. Erdmann J, Töpsch R, Lippl F, Gussmann P, Schudziarra V. Postprandial response of plasma ghrelin levels to various test meals in relation to food intake, plasma insulin and glucose. J Clin Endocrinol Metab 2004; 89: 3048–3054.
14. Nakai Y, Hosoda H, Nin K, Ooya C, Hayashi H, Akamizu T, Kangawa K. Short-term secretory regulation of the active form of ghrelin and total ghrelin during an oral glucose tolerance test in patients with anorexia nervosa. Eur J Endocrinol 2004; 150: 913–914.
15. Otto B, Tschöp M, Frühauf E, Heldwein W, Fichter M, Otto C, Cuntz U. Postprandial ghrelin release in anorectic patients before and after weight gain. Psychoneuroendocrinology 2005; 30: 577–581.
16. Shiiya T, Nakaqzato M, Mizuta M, Date Y, Mondal MSW, Tanaka M, Nozoe S, Hosoda H, Kangawa K, Matsukura S. Plasma ghrelin levels in lean and obese humans and the effect of glucose on ghrelin secretion. J Clin Endocrinol Metab 2002; 87: 240–244.
Labels
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain managementArticle was published in
Journal of Czech Physicians
Most read in this issue
- Drug treatment of obesity – current situation and perspectives
- hyperparathyroidism as a cause of hypercalcemia in a patient with breast cancer
- Relation between dietary and physical activity patterns and obesity in seven year old children – current situation – WHO study
- Relation between insulin secretion and action – study of genetic determination