Principle and Importance of Using the Array CGH in Hematooncology

Czech version

Authors: M. Jarošová; H. Pospíšilová; R. Plachý; M. Divoká; M. Holzerová; T. Papajík; J. Koptíková ¹; K. Indrák
Authors‘ workplace: Hemato-onkologická klinika LF a FN, Olomouc ; Centrum biostatistiky a analýz LF a PřF MU, Brno ¹
Published in: Čas. Lék. čes. 2006; 145: 9-13
Category: Review Article

Overview

Identification of chromosomal changes and variation in DNA copy number allows us to understand pathogenesis of tumors. To the frequently diagnosed chromosomal changes belong acquired gains and losses of chromosomal regions carring genes involved in cellular proliferation and differentiation as well as oncogenes and tumor suppressor genes. The determination of gene changes is limited by techniques used for their identification. The introduction of genom-wide microarray technology, resolution has rapidly increased. Array comparative genomic hybridization (arrayCGH) offers higher resolution for genome-wide detection of chromosomal alteration and it is able to analyze hundreds to thousands of genes presented on microarray in one experiment. The aim of this study was to perform arrayCGH technology and to stress its value for the identification of chromosomal imbalances in hematological malignancies.

Key words:
DNA, in situ hybridization, CGH, arrayCGH, microarray, oncogene, tumor suppressor gene.

Labels

Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental Hygienist

Some New Findings in the Pathogenesis of Myeloproliferative Disorders and New Insight into More Effective Treatment

Acylation Stimulating Protein – Its Role in Control of Metabolism in the Adipose Tissue

High Dose Therapy with Autologous Stem Cell Transplantation in Patients with Hodgkin’s Lymphoma: Long Term Follow-up in Patients Treated in One Center

Importance of Quantitative Evaluation of BCR-ABL Transcripts Using Real-time PCR for Effective Treatment of Chronic Myeloid Leukemia

Significance of Confirmatory Testing of HLA System in Unrelated Haematopoietic Stem Cell Donors

The Use of Quantitative Assessment of Wilms Tumour Gene for Monitoring of Residual Disease in Acute Myeloid Leukemia Patients

Acute Toxicity of High Dose Interstitial Brachytherapy Boost in Prostate Cancer

Familial Haemophagocytic Lymphohistiocytosis Caused by Perforin Deficit Can Be Successfully Treated by Haematopoietic Stem Cell Transplantation – The First Diagnosed Case in the Czech Republic

Hydatic Cysts – Echinococcosis

Abuse of Buprenorphine Becomes a Problem of the Czech Republic

Robotic Surgery

Impact Factor – Good Servant, but a Bad Master