Authors: M. Skleničková 1; J. Fajkus 1 Authors‘ workplace: Biofyzikální ústav AV ČR, Brno 2Laboratoř funkční genomiky a proteomiky MU, Brno 1 Published in: Čas. Lék. čes. 2003; : 479-482 Category:
Stable telomere maintenance is essential for the indefinite cellular proliferation of germline and tumour cells. Inmost cases, telomere synthesis is performed by nucleoprotein enzyme complex of telomerase, that results instabilisation of telomeres shortened to < 7 kb. Rarely, telomeres may be maintained via alternative (recombinationbased)mechanism, which produces telomeres of heterogenous lengths (3–50 kb). Analysis of telomeres by in situtechniques, such as fluorescent in situ hybridisation (FISH) on metaphase spreads or on extended DNA fibres(fiber-FISH) and Primed in situ labelling (PRINS), enables to distinguish between these two mechanisms and toanalyse individual telomeres in the given type of cells.
Key words: telomeres, FISH, fiber-FISH, PRINS.
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