Fecal calgranulin C in patients with inflammatory bowel disease

Czech version

Authors: K. Malíčková ¹; M. Bortlík ^2,3; D. Ďuricová ^2,4; M. Lukáš ^2,3; L. Hrdlička ²; N. Machková ²; M. Lukáš ^1,2
Authors‘ workplace: Ústav lékařské biochemie a laboratorní diagnostiky 1. LF UK a VFN, Praha ¹; Klinické a výzkumné centrum pro střevní záněty ISCARE I. V. F. a. s. a 1. LF UK, Praha ²; Interní klinika 1. LF UK a ÚVN, Praha ³; Farmakologický ústav, 1. LF UK, Praha ⁴
Published in: Gastroent Hepatol 2014; 68(1): 10-13
Category: IDB: Original Article

Overview

The aims of the study are the analysis of fecal calgranulin C levels in patients with bowel inflammation; comparison with the fecal calprotectin test; calculation of sensitivity and specificity for the diagnosis of inflammatory bowel disease (IBD); determination of the variability parameters of enzyme-linked immunosorbent assay (ELISA) for calgranulin C detection.

Materials and Methods:
Fifty fecal samples from patients with Crohn’s disease (n = 21), ulcerative colitis (n = 19) and unspecified bowel disorders (n = 10) were analysed. The fecal levels of calprotectin (MRP8/14) and calgranulin C (MRP6) were compared in the eluates. Statistical data analysis consisted of a quantitative and qualitative results comparison, a measurement of variability parameters and diagnostic performance of both tests. In calgranulin C the reference range of normal values was estimated.

Results:
A significant positive correlation between fecal calprotectin concentrations and calgranulin C was found (r = 0.829, p <0.001). During the qualitative analysis of the results, a method consensus was achieved in 90% of cases. Diagnostic sensitivity of fecal calgranulin C for diagnosis of IBD was 75%, and specificity reached 88%. Parameters of variability for fecal calgranulin C ELISA were in accordance with ISO15189 requirements.

Conclusion:
Calgranulin C is a new biomarker of intestinal inflammation. It shows significant correlation with fecal calprotectin but its level should be increased primarily in the course of a neutrophil-mediated inflammation.

Keywords:
calgranulin C – calprotectin – S100 proteins – inflammatory bowel disease

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE „uniform requirements“ for biomedical papers.

Submitted:
16. 9. 2013

Accepted:
9. 12. 2013

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