The Comparison of the Enzymatic and the Acid Hydrolyse at the Toxicological screening of the small amount of Urine
F. Vorel; M. Dědičová; J. Lázničková
Soudnělékařské oddělení Nemocnice České Budějovice, a. s.
Soud Lék., 51, 2006, No. 2, p. 27-29
The article deals with the toxicological screening directed at the identification and confirmation of the acid, neutral and basic medicaments and drugs in the urine. The efficiency of the hydrolysation using ß-glukuronidase (enzymatic hydrolyses) and using 0.1M hydrochloric acid (acid hydrolyses) from 2 ml of the urine were compared. The solid phase extraction (SPE) and the GC-MS identification were used.
The efficiency of the extraction of paracetamol, buprenorphine and morphine is better by means of the enzymatic hydrolyses. Vice-versa the acid hydrolyses appears to be more applicable for benzodiazepines. The acid hydrolyses is faster and cheaper and the extracts contain less of the ballast materials.
toxicological screening – medicaments, drugs – hydrolysis – SPE, GC-MS
Forensic medical examiner