Acquiring Microscopic Images of Very High Resolution by a Method ofComposing

Overview

Composing microscopic images of very high resolution from several parts posed some problems.One of them was the necessity to adjust the focusing level when moving from one part to another.Re-focusing lead to problems with joining the image parts, which did not correspond exactly, andthe area of image fusion was noticeable. A computer program was developed to overcome theseproblems. Our program worked with all the image parts together to find their optimal order forimage fusion. Individual image parts were joined using a steep gradient running along a randomlygenerated curve. This method gave good results even in images with background or holes in thetissue. The method of composing large images from individual parts was used for digitizing theskin lymphoma collection of the Institute of Dermatology, University Hospital, Zürich. This collectionof digital images is a part of the 6th version of Hypertext atlas of Dermatopathology atwww.muni.cz/atlases.

Key words:
digital microphotography - image analysis - interactive atlas - dermatopathology -hypertext