Time Relations of the Expression of Adhesive Proteins and Other Markersand the Age of Injuries

Czech version

Authors: J. Dreßler ^*; P. Strejc ^**; P. Klír ^***; E. Müller ^*; O. Boubelík ^**; I. Grossová ^**
Authors‘ workplace: Technická univerzita Drážďany, LF C. G. Carus, Ústav soudního lékařství, Drážďany **Univerzita Karlova, 1. LF UK, Ústav soudního lékařství a toxikologie, Praha ***Subkatedra soudního lékařství IPVZ, Praha ^*
Published in: Čes.-slov. Patol., , 2002, No. 3, p. 38-42
Category:

Overview

In addition to evidence of the vital reaction it is important from the forensic aspect to assess theage of wounds, in particular in wounds with a short time of survival.T o both can contributedetection of adhesive molecules, identified in recent years, possibly in combination with othermarkers of early stages of reparative inflammation.The submitted paper comprises the results ofinvestigation of 465 skin wounds.The investigated samples were obtained from necroptic material,excision from wounds of treated patients and from experimental injuries in mice.Ass essmentof the age of injuries by means of endothelial adhesive molecules was made in paraffin sectionsafter transfer into Varioclave and using the ABC techniqueref.. In human material a strong positivereaction of ICAM-1 was observed first after 1 and a half hours and latest after 3 and a half days,in P-selectin first after 3 mins., latest after 7 hours,in E-selectin first after 1 hour and latest after17 days, in VCAM-1 first after 3 hours and latest 3 and a half days after the development of theinjury.Expr ession of L-selectin was not typical for the injury.In skin injuries of mice positiveimunohistochemical reactions were found as a rule sooner than in skin injuries of humans. Fibronectinwas detected in paraffin sections from 70 skin wounds of dissected subjects immunohistochemicallyby the indirect immunoperoxidase reaction and by the use of the ABC techniquelabelled with alkaline phosphatase.P ositive evidence was observed first after 5 minutes andlatest after 8 hours.Concl usion: Detection ofa rise of expression of ICAM-1, VCAM-1 and P- andE.selectin and the formation of basic netlike fibronectin structures improves the assessment of theage of wounds with a short survival period.

Key words:
selectins - ICAM-1 - VCAM-1 - fibronectin - age of wounds - immunohistochemistry

Full text is not available online.

If interested in a scan of this journal, contact NTO ČLS JEP.