Immunophenotyping and other applications of flow cytometry in acute leukemias
Authors:
O. Hrušák; E. Mejstříková; V. Kanderová; M. Vášková; T. Kalina
Authors‘ workplace:
Klinika dětské hematologie a onkologie 2. LF UK a FN Motol, Praha, přednosta prof. MUDr. Jan Starý, DrSc.
Published in:
Čes-slov Pediat 2015; 70 (2): 85-91.
Category:
Acute leukemias in children
Overview
Cytometric immunophenotyping is one of the most powerful methods in acute leukemia diagnostics. Main advantages are its speed, reliability and knowledge of parameters investigated at a single-cell level. Disadvantages may include relative expensiveness compared to some basic methods. Cytometric immunophenotyping should be interpreted by an expert and reported to the clinical physician so that all relevant questions regarding the patient's classification and treatment are addressed adequately. Flow cytometry can evaluate other parameters such as the DNA contents or proteome analysis. Practical diagnostic usefulness of the newest generation multiparametric instruments is being assessed.
Key words:
acute leukemias, children, immunophenotyping, cytometry
Sources
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Neonatology Paediatrics General practitioner for children and adolescentsArticle was published in
Czech-Slovak Pediatrics
2015 Issue 2
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