The incidence of anaemia and the use of the ELISA method for measuring hepcidin levels in children with inflammatory bowel disease
Authors:
J. Houda 1; P. Džubák 1,2; E. Karásková 1; D. Holub 2; D. Vydra 1; D. Mlčůchová 2; M. Hajdúch 2; O. †pozler 3; D. Pospíšilová 1
Authors‘ workplace:
Dětská klinika při LF UP a FN, Olomoucpřednosta prof. MUDr. V. Mihál, CSc.
1; Laboratoř experimentální medicíny, Ústav molekulární a translační medicíny, Lékařská fakulta UP, Olomoucředitel doc. MUDr. M. Hajdúch, Ph. D.
2; Dětská klinika LF a FN, Hradec Královépřednosta prof. MUDr. M. Bayer, CSc.
3
Published in:
Čes-slov Pediat 2014; 69 (3): 137-147.
Category:
Original Papers
Overview
The aim of the study was to identify the incidence of anemia in children with inflammatory bowel disease (IBD) (n=133), to define characteristics of anemia and to assess the results of hepcidin measurement by ELISA test in selected group of IBD patients (n=56). Anemia was found in 47.7% of patients. The ferritin level was decreased and the soluble transferrin level was increased in 43% of anemic patients. These findings correspond with a combination of iron deficient anemia (IDA) and anemia of chronic diseases (ACD). A typical laboratory feature of ACD with increased ferritin level was present in only 7.7% of anemic patients. Mean value of hepcidin level in patients with IBD was 42.0 ng/ml (SD 34.1), which is in normal range delivered by ELISA kit manufacturer (13.3–54.4 ng/ml). The hepcidin levels were increased in 55% of the female IBD patients and in 18% of the male patients. The statistically significant positive correlation between hepcidin and CRP levels was found (R=0.30 and p<0.041). The hepcidin levels were significantly higher in young adults over 18 years old than in children aged <18 years.
Our findings suggest that ACD is the most common type of anemia in IBD children and it is accompanied with true iron deficiency. These results differ from currently published data in adult IBD patients. Measuring hepcidin levels is a helpful tool in iron deficiency diagnosis and may represent an important indicator when selecting the most appropriate treatment option. It may also help clarify the hierarchy of regulatory mechanisms in iron metabolism.
Key words:
anaemia of chronic diseases, iron deficiency anaemia, inflammatory bowel diseases, hepcidin, ferroportin, ELISA
Sources
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Neonatology Paediatrics General practitioner for children and adolescentsArticle was published in
Czech-Slovak Pediatrics
2014 Issue 3
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