Association of the -1082 G/A polymorphism in the IL-10 gene promoter with production of autoantibodies in rheumatoid arthritis


Authors: P. Němec 1;  M. Goldbergová-Pávková 2;  J. Gatterová 3;  Z. Fojtík 4;  A. Vašků 2;  M. Souček 1
Authors‘ workplace: Revmatologická ambulance, II. interní klinika, Fakultní nemocnice u sv. Anny v Brně a Lékařské fakulty Masarykovy univerzity, Brno, 2Ústav patologické fyziologie, Lékařské fakulty Masarykovy univerzity, Brno 1;  Revmatologický ústav, Praha, 4Revmatologická ambulance, Interní a hematoonkologická klinika Fakultní nemocnice Brno a Lékařské fakulty Masarykovy univerzity, Brno 3
Published in: Čes. Revmatol., 16, 2008, No. 3, p. 104-109.
Category: Original Papers

Overview

Interleukin-10 (IL-10) is an immunoregulatory cytokine, usually considered to mediate downregulation of the inflammatory response in rheumatoid arthritis (RA). Some effects of IL-10 are not anti-inflammatory, for example activation of B cells to promote autoantibody production. Allelic polymorphisms located in the promoter region of IL-10 gene may contribute to the regulation of autoantibodies production. To examine the association between both individual IL-10 single nucleotide polymorphism -1082 G/A with the production of rheumatoid factors (RFs) in RA patients. A total of 151 patients with RA diagnosed according to the revised criteria of the American College of Rheumatology for RA were consecutively recruited into the study. All patients had a disease duration from the first manifestation of the disease symptoms of at least 24 months. Polymerase chain reaction amplification was used for analysis of the promoter polymorphism of the IL-10 gene. Serum levels of RFs were measured by ELISA. We observed significant differences in genotype distribution of the -1082 G/A polymorphism between IgM RF, IgA RF, and IgG RF positive/negative subgroups of RA patients, with higher prevalence of the GG genotype within IgM RF (Pg = 0.006), IgA RF (Pg = 0.05), and IgG RF (Pg = 0.007) negative RA patients. Results obtained in this study provide the evidence of an association of the -1082 G/A polymorphism in IL-10 gene promoter with the production of RFs, which are associated with poorer prognosis of RA.

Key words:
autoantibody, gene, interleukin-10, polymorphism, rheumatoid arthritis, rheumatoid factor


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Labels
Dermatology & STDs Paediatric rheumatology Rheumatology
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