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Inactivation of BRCA1, BRCA2 and p53 Genes in Sporadic Ovarian Cancer


Authors: M. Zikán 1;  P. Pohlreich 2;  P. Freitag 1;  M. Janoušek 1;  D. Pavlišta 1;  D. Fischerová 1;  N. Jančárková 1;  J. Sláma 1;  I. Pinkavová 1;  D. Cibula 1
Authors‘ workplace: Onkogynekologické centrum, Gynekologicko-porodnická klinika 1. LF UK a VFN, Praha, přednosta prof. MUDr. A. Martan, DrSc. 1;  Ústav biochemie a experimentální onkologie 1. LF UK, přednosta prof. MUDr. A. Šedo, DrSc. 2
Published in: Ceska Gynekol 2008; 73(5): 298-302
Category: Original Article

Overview

Objective:
To analyze loss of heterozygosity (LOH), loss of expression and somatic mutations of BRCA1, BRCA2 and p53 genes in sporadic epithelial ovarian cancer samples.

Design:
Original paper.

Setting:
Oncogynecologic center, Clinic of Obstetrics and gynecology, First Faculty of Medicine, Charles University in Prague and General Teaching Hospital, Prague.

Material and methods:
We used genomic DNA and total RNA from peripheral blood and fresh frozen tumor as a template for LOH, loss-of-expression and mutation analyses.

Results:
LOH in at least one region was found in 60% of tumors. Majority of these alterations occurred not solely, but in conjunction with other region deletions.

Conclusion:
Our study confirms high frequency of somatic alteration of BRCA1, BRCA2 and p53 genes in sporadic epithelial ovarian cancer.

Key words:
BRCA1, BRCA2, p53, ovarian cancer, LOH.


Sources

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Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine
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