Authors: K. Veselá; G. Tauwinklová; P. Trávník; J. Veselý; S. Hlaváčová; D. Zudová; O. Řezáčová; R. Rybář; J. Rubeš Authors‘ workplace: Sanatorium REPROMEDA, Brno, vedoucí lékař MUDr. J. Veselý, CSc. 2Výzkumný ústav veterinárního lékařství Brno, ředitel MVDr. M. Toman, CSc. 1 Published in: Ceska Gynekol 2003; (2): 89-94 Category:
Objective: Presentation of preimplantation genetic diagnosis and the set of laboratory processes likeaspiration, preparation and evaluation of polar bodies, sperm cells and blastomeres using FISHmethod (fluorescent in situ hybridization) in ART.Design: Review.Setting: Sanatorium REPROMEDA, Brno, Veterinary Research Institute, Brno.Methods: Overview of published data and own clinical experience with the cell aspiration methods,evaluated sample preparation and the proper chromosomes visualisation using FISH method.Conclusion: The review brings an overview of conditions and methods including sample obtaining,FISH analysis preparation and implementation, processed during PGD.
Key words: preimplantation genetic diagnosis, PGD, FISH, genetic factor, infertility
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