Fructose and Glucose in Follicular Fluid and Serumof IVF Patients

Overview

Objective:
To find out if there is some amount of fructose in the follicular fluid of IVF stimulatedpatients.I so then to compare fructose and glucose levels with IVF outcome,type of stimulationand infertility factors.Design:Retrospective study.Setting:Department of Gynaecology and Obstetrics,Charles University,the Czech Republic.Methods:202 women undergoing IVF treatment since 11/1999 till 02/2001 were evaluated.Wemeasured a level of fructose and glucose in a sample of serum and follicular fluid (FF)obtained atovum pick-up.Then the results were compared with the IVF outcome,the length of stimulationand infertility factors.The control group consists of 23 women with a pure andrologic factor ofinfertility.Results:At the day of pick-up the serum concentration of glucose and fructose taken on an emptystomach is on average 5.43 ±1.35 mmol/l and 25.05 ±9.26 m ml compared to 5.26 ±1.00 mmol/l and23.69 ±m ml in control group.The concentration of both glucose and fructose in FF correlateswith their serum level.The FF concentration of glucose is on average 3.75 ±1.32 mmol/l (70%oserum concentration)and fructose 11.34 ±5.29 m ml (48%of serum concentration)by comparisonwith 3.78 ± 0.91 mmol//l (73 %)and 12.37 ±7.06 m ml (53%)in control group.Conclusion:There is a possible influence of follicular fluid saccharides like glucose and fructosein process of fertilization.We derived this hypothesis from studies evaluating the role of sacchari-des in endometrial secretion,cervical mucous and seminal plasma.We assumed that patients witha successful IVF cycle have a lower fructose concentration in FF.The clinical pregnancy is reallymildly lower but this difference is not statistically significant.We confirmed FF fructose is inclose correlation with its serum concentration.But we did not confirm the FF glucose and FFfructose concentration is related to the IVF outcome.

Key words:
fructose,glucose,follicular fluid,IVF