Effect of CdCl2 and CuSO4 on the Production of Flavonoids by the Culture of Ononis arvensis L. in vitro

Overview

The effect of the abiotic elicitors CuSO4 and CdCl2 on the production of flavonoids in the callus culture of Ononis arvensis after 24; 48 and 168-hour administration was examined. The tested elicitors markedly influenced the production of flavonoids in this culture. A statistically significant increase in the production of flavonoids was recorded with the use of a CdCl2 solution in a concen- tration of 0.5 mg.l -1 and a concentration of 0.05 mg.l -1 after 48 hours of cultivation, in a concentration of 0.005 mg.l -1 after 24 and 48 hours of cultivation. In a CuSO4 solution in a concentration of 0.5 mg.l -1 it was after both 48 and 168 hours. The highest increase in the level of flavonoids was found after elicitation of CdCl2 in a concentration of 0.005 mg–l -1

Key words:
Ononis arvensis – flavonoids – elicitation – heavy metals