Authors: J. Stano 1; I. Šafařík 2; P. Kovács 3; K. Neubert 4; M. Šafaříková 2 Authors‘ workplace: Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava 2 Laboratoř biochemie a biotechnologie Ústavu ekologie krajiny České akademie věd, České Budějovice 3 Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty 1 Published in: Čes. slov. Farm., 1998; , 271-272 Category:
By means of an insoluble chromogenic substrate (black gelasphere), a simple, sensitive and repro - ducible method of detection of extracellular proteolytic enzymes was worked out. Extracellular proteolytic enzymes (produced by a callus or suspension culture) hydrolyse black gelasphere. Colouring around the callus culture and under it is a sign of the activity of extracellular proteolytic enzymes produced by the callus culture. The intensity of colouring of the cultivating medium is a measure of the activity of extracellular proteolytic enzymes of the suspension culture.
Key words: extracellular proteolytic enzymes – insoluble chromogenic substrate – black gelasphere – Chelidonium majus L.
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