Immunohistochemical Analysis of Pars Tensa Retraction Pocket in Children
Authors:
M. Urík 1,2; P. Hurník 3,4; D. Žiak 3,4; J. Macháč 1,2; I. Šlapák 1,2; O. Motyka 5; J. Dvořáčková 3; P. Jabandžiev 6
Authors‘ workplace:
Klinika dětské otorinolaryngologie, Lékařská fakulta, Masarykova univerzita, Brno
1; Fakultní nemocnice Brno
2; Ústav patologie, Lékařská fakulta, Ostravská univerzita, Ostrava a FN Ostrava
3; CGB laboratoře, Ostrava, Vítkovice
4; Centrum nanotechnologií, VŠB-Technická univerzita, Ostrava
5; Pediatrická klinika LF MU a FN Brno
6
Published in:
Otorinolaryngol Foniatr, 66, 2017, No. 4, pp. 198-204.
Category:
Original Article
Overview
Introduction:
Immunohistochemical analysis of pars tensa retraction pocket in children. Identification of signs typical for cholesteatoma and to support the retraction theory of cholesteatoma.
Methods:
Standard process of immunohistochemistry of retraction pockets. For immunohistochemistry we used CD45 LCA (Leukocyte common antigen), CD31 (platelet and endothelial cell adhesion molecule), D2-40 (podoplanin - lymphatic vessels) MMP9 (matrix metalloproteinase 9 - degradation of extracellular matrix) Ki67 (nuclear proliferation).
Results:
In all parameters, except for MMP9, we observed a significantly higher incidence in stage III retraction pockets than at II.
Conclusion:
We have described the immunohistochemical features of the pars tensa retraction pocket of the ear drum in children leading to cholesteatoma. All observed signs occur in the structure of the matrix and perimatrix cholesteatoma. For all monitored parameters except for MMP9, we showed a significantly higher incidence in stage III retraction pockets than in stage II. This observation indicates that the retraction pocket is a progressive disease and that it is a precholesteatoma stage.
KEYWORDS:
retraction pocket, immunohistochemical analysis, cholesteatoma, childhood
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Audiology Paediatric ENT ENT (Otorhinolaryngology)Article was published in
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