Evaluation and applicability of nonbiopsy criteria in children and adolescents according to ESPGHAN for diagnosis of celiac disease


Authors: J. Kabátová 1,2;  R. Husťak 2,3;  S. Blažíčková 2;  V. Bošák 2
Authors‘ workplace: Gastroenterologická ambulancia, Odborná detská ambulancia, s. r. o., Piešťany, Slovenská republika 1;  Katedra laboratórnych vyšetrovacích metód v zdravotníctve, FZaSPTU v Trnave, Slovenská republika 2;  1. LF UK v Praze 3
Published in: Gastroent Hepatol 2017; 71(6): 469-475
Category:
doi: 10.14735/amgh2017csgh.info07

Overview

Summary:
The paper focuses on the analysis of anti-tissue transglutaminase 2 (anti-TG2) antibodies and endomysial antibodies (EMA) and HLA-DQ2/HLA-DQ8 haplotypes present in symptomatic children and adolescents with suspected coeliac disease. This refers to applying the new 2012 ESPGHAN criteria allowing to make the diagnosis of coeliac disease without an enterobiopsy. A total of 258 children and adolescents (86 males and 172 females), aged 2–18 years, were retrospectively examined to determine the performance of coeliac disease testing according to national guidelines from 2009. The obtained data were applied according to the revised 2012 ESPGHAN criteria allowing to make the diagnosis of coeliac disease in indicated cases without an anterobiopsy. The nonbiopsy criteria revealed that 33.3% (86) of symptomatic children and adolescents in our cohort with high anti-TG2 titers and positive EMA could have been initially diagnosed without an intestinal biopsy. All patients presented with advanced intestinal atrophy Marsh 2–3. Part of the rationale of the study was to determine the sensitivity and specificity, the positive and negative predictive values of the final laboratory tests, and the diagnostic accuracy of the combined tests using antibodies.

Key words:
coeliac disease – ESPGHAN – anti-TG2 – EMA – HLA-DQ2/HLA-DQ8

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE „uniform requirements“ for biomedical papers.

Submitted:
21. 3. 2017

Accepted:
24. 4. 2017


Sources

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Labels
Paediatric gastroenterology Gastroenterology and hepatology Surgery
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