Prevalence and characteristics of thermotolerant Campylobacter spp. in the human food chain

Czech version

Authors: J. Bardoň ^1,2,3; I. Koláčková ⁴; V. Husičková ¹; M. Röderová ^1,3; R. Karpíšková ^4,5; T. Štosová ⁶; M. Kolář ^1,3
Authors‘ workplace: Ústav mikrobiologie, Lékařská fakulta UP v Olomouci ¹; Státní veterinární ústav Olomouc ²; Ústav molekulární a translační medicíny, Lékařská fakulta UP v Olomouci ³; Výzkumný ústav veterinárního lékařství, v. v. i. Brno ⁴; Ústav hygieny a technologie mléka, Fakulta veterinární hygieny a ekologie, VFU Brno ⁵; Fakultní nemocnice Olomouc ⁶
Published in: Epidemiol. Mikrobiol. Imunol. 63, 2014, č. 3, s. 232-237
Category: Review articles, original papers, case report

Overview

Objectives:
To monitor the prevalence of Campylobacter spp. in poultry in slaughterhouses, poultry and pork liver at retail, and cow’s milk in Moravia. To determine the resistance of animal isolates to selected antibiotics; and to compare it with an antibiogram of human strains.

Material and Methods:
Throughout the year 2013, the following samples were collected in the South Moravian and Olomouc Regions: mixed samples of broiler cecal contents in slaughterhouses, fresh and frozen chickens and pork liver at retail, and raw cow’s milk from vending machines. The samples were both qualitatively and quantitatively analyzed for the presence of Campylobacter spp. The isolates recovered were tested for resistance to antibiotics. For comparison, antimicrobial resistance was also studied in human isolates from the same regions.

Results:
A total of 41.8% of the tested food samples were found to contain Campylobacter spp.. The most contaminated (73.2%) were fresh chickens. Campylobacter spp. were not detected in raw cow’s milk samples. The isolates showed high levels of resistance to quinolone antibiotics and, in the case of C. coli, also to tetracycline and streptomycin.

Conclusion:
The studied commodities were frequently contaminated with Campylobacter spp. The levels of contamination (in CFU/g) varied between commodities and so, evidently, did the real risk for human infections. When antibiotic therapy is needed, quinolone antibiotics cannot be used. Adherence to high standards of consumer safe food handling is crucial for the prevention of diseases.

Keywords:
Campylobacter spp. – prevalence – food – resistance to antibiotics

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