Identification of Dermatophytes Using MALDI-TOF Mass Spectrometry


Authors: R. Walková 1,2;  H. Janouškovcová 1,2;  M. Šnajdrová 1;  V. Hubka 3,4;  A. Čmoková 3,4;  J. Hrabák 1,2
Authors‘ workplace: Ústav mikrobiologie, Fakultní nemocnice Plzeň, přednosta RNDr. Karel Fajfrlík, Ph. D. 1;  Laboratoř antibiotické rezistence a aplikací hmotnostní spektrometrie v mikrobiologii, Biomedicínské centrum, Lékařská fakulta v Plzni, Univerzita Karlova, Plzeň, vedoucí laboratoře Costas C. Papagiannitsis, Ph. D. 2;  Katedra botaniky, Přírodovědecká fakulta, Univerzita Karlova, Praha, vedoucí katedry doc. RNDr. Yvonne Němcová, Ph. D. 3;  Laboratoř genetiky a metabolismu hub, Mikrobiologický ústav, Akademie věd České republiky, v. v. i., Praha, vedoucí laboratoře Mgr. Miroslav Kolařík, Ph. D. 4
Published in: Čes-slov Derm, 93, 2018, No. 6, p. 250-256
Category: Clinical and laboratory Research

Overview

Dermatophytes are microscopic fungi causing skin infections and infections of skin adnexa. Their identification is usually performed by morphological characteristics requiring experienced microbiologists and quiet long turnaround time. In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been introducing to many diagnostic laboratories allowing identification of dermatophytes as well. For that purpose, however, specific procedures comparing with bacteria and yeasts must be used because of their complex morphology and specific cultivation requirements. This study is focused on comparison of MALDI-TOF MS-based identification, identification using DNA sequencing of Internal Transcribed Spacer (ITS) rDNA region and conventional methods. In the study, 81 clinical isolates of dermatophytes were included. MALDI-TOF MS correctly identified 74% of all isolates. Using classical methods, only 47% of isolates were identified. Thus, MALDI-TOF MS provides a useful tool for identification of those fungi in routine microbiological laboratories.

Keywords:

MALDI Biotyper – Nannizzia – Trichophyton


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Dermatology & STDs Paediatric dermatology & STDs
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