Authors: P. Drbohlav 1; Simona Jirsová 1; J. Mašata 1; L. Jech 2; V. Bencko 3; M. Omelka 4; J. Zvárová 4 Authors‘ workplace: Gynekologicko-porodnická klinika 1. LF UK a VFN, Praha, přednosta prof. MUDr. A. Martan, DrSc. 1; AXYS Varilab s. r. o., Vrané nad Vltavou, vedoucí laboratoře RNDr. L. Jech, CSc. 2; Ústav hygieny a epidemiologie 1. LF UK, Praha, přednosta prof. MUDr. V. Bencko, DrSc. 3; Euromise centrum, Karlova univerzita a Akademie věd, Praha, ředitelka prof. RNDr. J. Zvárová, DrSc. 4 Published in: Ceska Gynekol 2005; 70(5): 377-383 Category: Original Article
Objective: The aim of this study was to confirm the possibility of detection of toxic polychlorinated biphenyls in blood and follicular fluid of infertile women undergoing IVF+ET program and determine the levels of some congeners. To confirm their cumulation in follicular fluid.
Design: Pilot study.
Setting: Department of Obstetrics and Gynecology, 1st Faculty of Medicine, Charles University and General Faculty Hospital, Prague, AXYS Varilab s.r.o., Vrané nad Vltavou, Institute of Hygieny and Epidemiology, 1st Faculty of Medicine Charles University and General Faculty Hospital, Prague, Euromise Centrum, Charles University, Prague.
Methods: We detected the level of polychlorinated biphenyls in blood and follicular fluid of infertile women undergoing IVF+ET program. We recieved the follicular fluid by transvaginal punction of follicles under ultrasonography control. The blood was taken before begining of anestezia. The follicular fluid and blood were frozen and transported to the laboratory. The samples were examined there by methods of gas chromatography and mass spectrometry. We were able to find all PCBs with 3–7 atoms of chlorine.
Results: We confirmed the possibility of detection of polychlorinated biphenyls (PCBs) in blood and follicular fluid of infertile women. We examineted the levels of PCB 77, 81, 105, 114, 118+123, 126, 156, 157, 167, 169, 189. The levels of PCBs were in ng/gram of fat. The levels of polychlorinated biphenyls differed from 0 to 400 ng/g of fat. Statistical analysis was made by t test a Wilcox test. All PCBs are cumulated in follicular fluid, except of PCB 126.
Conclusion: The possibility of detection of PCBs in blood and follicular fluid of infertile women undergoing IVF+ET program was confirmed. The cumulation of these xenobiotics in follicular fluid was found. In the future we will analyse the relationship between the presence of these xenobiotics and achieving succesful pregnancy.
Key words: IVF+ET program, polychlorinated biphenyls, kongeners, xenobiotics
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