Authors: D. Koryntová; L. Jelínková; K. Řežábek; J. Živný Authors‘ workplace: Gynek. -porod. klinika 1. LF UK a VFN, Praha, přednosta prof. MUDr. J. Živný, DrSc. Published in: Ceska Gynekol 1999; (6): 383-388 Category:
Design: The literature on intrauterine insemination, IVF, ICSI, MESA/TESE, preimplantation ge-netic diagnosis, cryopreservation, in vitro maturation, cloning and stem cells.Setting: Assisted Reproduction Centre, Ist Medical Faculty of Charles University Prague andGeneral Hospital Prague, Apolinářská 18, 128 00, Prague 2, Czech Republic.Results: IUI, IVF, ICSI also in combination with MESA/TESE are widely used relatively higheffective procedures. According to Czech Republic Assisted Reproduction Registry, the pregnan-cy rate per transfer using IVF, ICSI is 25%, 30% respectively. Cryopreservation of sperm andembryos is a basic part of assisted reproduction, the resulst with oocytes freezing are still notsatisfactory. Preimplantation genetic diagnosis is performed in few countries only due to techni-cal barriers and ethical restrictions. Other methods like cloning, nuclear transfer, embryotherapyor gene manipulation using embryonic stem cells are still only subjects of research.Conclusion: During the last ten years, assisted reproduction showed rapid development in a num-ber of treated couples and the spectrum of used methods changed radically.
Key words: assisted reproduction methods, IVF, ICSI, MESA/TESE, cryopreservation, cloning, em-bryotherapy, embryonic stem cells
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