Oral Carriage of Staphylococcus aureus among Dental Patients in Dependence on Conditions in Oral Cavity
(Original Article – Retrospective Study)
Authors:
A. Kaiglová 1; J. Špajdelová 1; M. Stanko 2; E. Dubajová 2
Authors‘ workplace:
Katedra laboratórnych vyšetrovacích metód v zdravotníctve, Fakulta zdravotníctva a sociálnej práce, Trnavská univerzita, Trnava
1; Zubná ambulancia LUXDENT, s. r. o., Nitra
2
Published in:
Česká stomatologie / Praktické zubní lékařství, ročník 116, 2016, 1, s. 12-19
Category:
Original articles
Overview
Introduction and aim:
Staphylococcus aureus is a significant opportunistic pathogen possessing highly variable genome, causing thus wide range of infections. An important location where this pathogen may occur in healthy individuals includes not only organs such as skin, pharynx, nasal mucosa, etc., but also oral cavity. The main objective of our study was to find out whether various oral cavity conditions such as periodontitis, fixed and removable dental prostheses and stomatological diseases with indications for extraction influence the level of oral cavity colonization by the Staphylococcus aureus, as well as to define the frequency of occurence of methicillin-resistant strains of this microorganism in stomatological patients.
Methods:
The examined collection consisted of 96 patients, who attended dental services LUXDENT, Ltd., in Nitra from June 2014 to January 2015. Patients were divided into the groups studied, i.e., the group of patients with 1. periodontitis, 2. dental diseases with indications for extraction, 3. fixed or removable prosthetic restorations and 4. control group. Microbiological samples were obtained by sterile swabs from oral and nasal cavity of subjects included into study and delivered to the laboratory within 24 h. At the laboratory, samples were processed by standard methods for isolation and identification of Staphylococcus aureus and confirmed by polymerase chain reaction, in which the specific region of nuc gene, characteristic for Staphylococcus aureus was amplified. Methicillin-resistant strains of Staphylococcus aureus were identified using disc difusion method according to the methodology of the European Committee on Antimicrobial Susceptibility Testing [8].
Results:
The presence of S. aureus was found in 35 samples (20.2%) out of 173 examined samples (oral and nasal cavity swabs), while 31 of those samples were methicillin-sensitive (88.58%) and four of them were methicillin-resistant (11.42%). There was a statistically important difference between the occurrence of the microorganism in nose and mouth (p < 0,05) indicating a more frequent occurrence in the nasal cavity. The presence of the microorganism in both nasal and oral cavities simultaneously was discovered only in three out of 96 patients (3.12%). When comparing the group of patients with fixed and removable prostheses to other groups, the occurrence of microorganism studied was significantly higher (p < 0,05).
Conclusions:
Our results indicate that the oral cavity may be an important site of Staphylococcus aureus colonisation and the conditions in the oral cavity, e.g., fixed and removable restorations may promote oral carriage of this microorganism.
Keywords:
Staphylococcus aureus – methicillin resistant Staphylococcus aureus – oral cavity – parodontitis – dental prostheses
Sources
1. Ammerlaan, H. S., Kluytmans, J. A., Wertheim, H. F., Nouwen, J. L., Bonten, M. J.: Eradication of methicillin-resistant Staphylococcus aureus carriage: a systematic review. Clin. Infect. Dis., roč. 48, 2009, č. 7, s. 922–930.
2. Blomqvist, S., Leonhardt, A., Arirachakaran, P., Carlen, A., Dahlén, G.: Phenotype, genotype, and antibiotic susceptibility of Swedish and Thai oral isolates of Staphylococcus aureus. J. Oral Microbiol. [online]. 2015, 7 [cit. 2015-08-15]. Dostupný z WWW: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409631/.
3. Brakstad, G. O., Aasbakk, K., Maeland, A. J.: Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J. Clin. Microbiol., roč. 30, 1992, č. 7, s. 1654–1660.
4. Buonavoglia, A., Latronico, F., Greco, M. F., D‘Abramo, M., Marinaro, M., Mangini, F., Corrente, M.: Methicillin-resistant staphylococci carriage in the oral cavity: a study conducted in Bari (Italy). Oral Dis. [online], roč. 16, 2010, č. 5, s. 465–468 [cit. 2015-08-15]. Dostupný z WWW: http://www.ncbi.nlm.nih.gov/pubmed/20233317.
5. Clinical and Laboratory Standards Institute: NCCLS Performance standards for Antimicrobial disc diffusion tests; Approved standards. 9th ed. CLSI Document M2-M9. Wayne, P.: Clinical and Laboratory Standards Institute; 2006.
6. Nozaki, C., Masaki, T., Kim, S. J., Cruz, R. S., Bermido, C. M., Kim, K. Y., Park, C.: Comparative prevalence of community-acquired-methicillin-resistant Staphyloccocus aureus (CA-MRSA) among Students of Centro Escolar University (Philippines), Kumamoto Health Science University (Japan) and Daegu Health College (Korea). Biomed. Res. [online], roč. 26, 2015, s. 259, cit. 2015-08-15]. Dostupný z WWW: http://www.biomedres.info/yahoo_site_admin/assets/docs/259-265.64225011.pdf.
7. Di Giacobbe, A., Pecetta, S., Virga, A., Scazzocchio, F., Aquilanti, L., Iebba, V., Passariello, C.: Diffusion of meticillin-resistant Staphylococcus aureus USA300 strains in central Italy. Int J. Antimicrob. Agents, roč. 37, 2010, č.4, s. 49–50.
8. European Committee on Antibacteral Susceptibility Testing: EUCAST: AST of bacteria. [on line]. 2014 [cit. 2015-04-01]. Dostupný z WWW: http://www.eucast.org/ast_of_bacteria/ .
9. Fomda, A. B. A., Thokar, M. A, Khan, A., Bhat, J. A., Zahoor, D., Bashir, G., Majid, A., Ray, P.: Nasal carriage of methicilin-resistant Staphylococcus aureus among healthy population of Kashmir. Indian J. Med. Microbiol., roč. 21, 2014, č. 13, s. 39–43.
10. Heo, S. M., Choi, K. S., Kazim, L. A., Reddy, M. S, Haase, E. M., Scannapieco, F. A., Ruhl, S.: Host defense proteins derived from human saliva bind to Staphylococcus aureus. Infect. Immun., roč. 81, 2013, č. 4, s. 1364–1373.
11. Kasmi, G., Refatllari, E., Dumani, S., Refatllari, A.: Early infective endocarditis due to Staphylococcus aureus following dental procedures. Clin. Lab., roč. 60, 2014, č. 11, s. 1933–1936.
12. Kaur, D. C., Narayan, P. A.: Mupirocin resistance in nasal carriage of Staphylococcus aureus among healthcare workers of a tertiary care rural hospital. Indian J. Crit. Care Med., roč. 18, 2014, č. 11, s. 716–721.
13. Kukita, K., Kawada-Matsuo, M., Oho, T., Nagatomo, M., Oogai, Y., Hashimoto, M., Suda, Y., Tanaka, T., Komatsuzawa, H.: Staphylococcus aureus SasA is responsible for binding to the salivary agglutinin gp 340, derived from human saliva. Infect. Immun., roč. 81, 2013, č. 14, s. 1870–1879.
14. Martin, M. V., Hardy, P.: Two cases of oral infection by methicillin-resistant Staphylococcus aureus. Brit. Dent. J., roč. 170, 1991, č. 2, s. 63–64.
15. Mehraj, J., Akmatov, M. K., Strompl, J., Gatzemeier, A., Layer, F., Werner, G., Pieper, D. H., Medina, E., Witte, W., Pessler, F., Krause, G.: Methicillin-sensitive and methicillin-resistant Staphylococcus aureus nasal carriage in a random sample of non-hospitalized adult population in northern Germany. PLoS One, roč. 9, 2014, č. 9, s. 24–29.
16. McCormack, M. G., Smith, A. J., Akram, A. N., Jackson, M., Robertson, D., Edwards, G.: Staphylococcus aureus and the oral cavity: An overlooked source of carriage and infection? Am. J. Infect. Control, roč. 43, 2015, č. 1, s. 35–37.
17. Merghni, A., Nejma, B. M., Hentati, H., Mahjoub, A., Mastouri, M.: Adhesive properties and extracellular enzymatic activity of Staphylococcus aureus strains isolated from oral cavity. Microb. Pathog., roč. 73, 2014, s. 7–12.
18. Moran, G. J., Krishnadasan, A., Gorwitz, R. J., Fosheim, G. E., Mcdougal, L. K., Carey, R. B., Talan, D. A.: Methicillin-resistant S. aureus infections among patients in the emergency department. N. Engl. J. Med., roč. 355, 2006, s. 666–674.
19. Nilsson, P., Ripa, T.: Staphylococcus aureus throat colonization is more frequent than colonization in the anterior nares. J. Clin. Microbiol., roč. 44, 2006, č. 9, s. 3334–3339.
20. Passariello, C., Puttini, M., Iebba, V., Pera, P., Gigola, P.: Influence of oral conditions on colonization by highly toxigenic Staphylococcus aureus strains. Oral Dis., roč. 18, 2012, č. 4, s. 402–409.
21. Petti, S., Polimeni, A.: Risk of methicillin-resistant Staphylococcus aureus transmision in the dental healthcare setting: a narrative review. Infect. Control. Hosp. Epidemiol., roč. 32, 2011, č. 11, s. 1109–1115.
22. Pharma-reports: Klinicky významné bakterie. 1. vyd., Praha, Triton, 2012, 128 s. ISBN 978-80 -7387-588-6.
23. Shetty, S. M., Shenoy, P. R., George, T. V.: Role of self care practices and denture maintenance in oral health, J. Orofac. Res., roč. 4, 2014, č. 1, s. 7–11.
24. Shetty, V., Trumbull, K., Hegde, A., Shenoy, V., Prabhu, R. K. S., Palavecino, E., Shetty, A. K.: Prevalence of community-acquired methicillin-resistant Staphylococcus aureus nasal colonization among children. J. Clin. Diagn. Res. [online], roč. 8, 2014, č. 12, s. 12–15 [cit. 2015-08-02]. Dostupný z WWW: http://www.ncbi.nlm.nih.gov/pubmed/25653946.
25. Kim, S. J., Park, C.: Panton-valentine leukocidin and staphylococcal cassette chromosome (SSCmec) from CA-MRSA (Community-Acquired Methicillin Resistant Staphylococcus aureus). Biomed. Res. [online], roč. 24, 2014, č. 4, s. 441–444 [cit. 2015-08-02]. Dostupný z WWW: http://www.biomedres.info/yahoo_site_admin/assets/docs/441-444-Kim-Cheolin.253134134.pdf.
26. Von Specht, M. H., Gardella, N., Ubeda, C., Grenon, S., Gutkind, G., Mollerach, M.: Community-associated methicillin-resistant Staphylococcus aureus skin and soft tissue infections in a pediatric hospital in Argentina. J. Infect. Dev. Ctries [online], roč. 8, 2014, č. 9, s. 1119–1128 [cit. 2015-08-01]. Dostupné z WWW: http://www.jidc.org/index.php/journal/article/view/4271.
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Maxillofacial surgery Orthodontics Dental medicineArticle was published in
Czech Dental Journal
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