Isolation of Immunoglobulins by Thiophilic Adsorption Chromatography

Overview

The separation of antibodies is a process of great importance. The present paper deals with themethod of thiophilic adsorption chromatography (TAC) based on the thiophilic sorbents. Thiophilicsorbents can interact with the immunoglobulins very selectively. Binding of immunoglobulins isdependent on the high ionic strength of the medium. Sorbed immunoglobulins are released by theionic strength decay. TAC provides immunoglobulins from the plasma (or other sources) in one step.Purity of immunoglobulins is comparable with results of the other methods. Benefits of TAC aredelicacy to immunoglobulins, simple handling and low costs of separation. By means of TAC it ispossible to eliminate immunoglobulins from biological materials. Thiophilic sorbents are currentlyavailable but the possibilities of their exploitation are practically unknown.

Key words:
antibodies – immunoglobulins – thiophilic adsorption –chromatography