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Intraocular Pressure after Instillation of Amino Acid Arginine and Combination of Two Antiglaucomatics (Trusopt + Xalatane Mixture) in Rabbits


Authors: Z. Oláh;  J. Veselovský;  A. Veselá
Authors‘ workplace: Klinika oftalmológie LF UK, Bratislava, prednosta prof. MUDr. P. Strmeň, CSc.
Published in: Čes. a slov. Oftal., 64, 2008, No. 4, p. 131-134

Overview

Purpose:
To evaluate the effect of instilled amino acid L-arginine.HCl and combination of two antiglaucomatics (Trusopt with Xalatane mixture) on the physiological intraocular pressure IOP in rabbits.

Methods:
Into the left conjunctival sac of 5 female rabbits of the New Zealand White species was instilled the mixture of 2 % Trusopt (Dorsolamidi hydrochlorici) with 0.005 % Xalatane (Latano-prostum) and after the one week break, the 10 % L-arginine.HCl in 2% Trusopt with 0.005% Xalatane mixture. The IOPs were measured in 0, 5, 15, 30, 60, 120, 180, 240 min. and 24 hours after the instillation. The right eyes were used as controls.

Results:
1. The combination of two antiglaucomatics in comparison with the control eye decreased significantly the IOP value already after 15 min. The major decrease of the IOP (6.5 mm Hg) was observed after 240 min. After 24 hours, the effectiveness was not significant. 2. After instillation of the 10 % L-arginine.HCl in 2% Trusopt with 0.005 % Xalatane mixture, during the next 24 hours, the biphasic decrease of the IOP was established. The maximum of the effectiveness of this mixture is after 30 min (decrease by 5.1 mm Hg). The evidence of the effect was present also after 24 hour (decrease by 2.5 mm Hg). The mean value of the IOP of the control eyes in both groups of experiments in course of 24 hours was stable and was at the initial values.

Conclusions:
The combination of two antiglaucomatics (Trusopt with Xalatane mixture) was used. The effect of those two substances is not separate or additive but acts as a newly formed substance. In the structures of the eye, this new substance has only poor interaction with the free amino acids of the conjunctival sac in vivo. After in vitro interaction of the L-arginine.HCl and combination of two antiglaucomatics (Trusopt with Xalatane mixture), a new bioactive substance with stronger effect on physiologic IOP values was produced.

Key words:
physiologic values of the IOP in rabbits; changes of the IOP after 10 % L-arginine.HCl in 2 % Trusopt with 0.005 % Xalatane mixture


Sources

1. Boyle, J.E., Ghosh, K., Gieser, D.K. et al.: A randomized trial comparing the dorzolamide-timolol combination given twice daily to monotherapy with timolol and dorzolamide. Ophthalmol., 105, 1998: 1945–1951.

2. Brubaker, R.F., Ingram, C.J., Schoff, E.O. et al.: C.B.: Comparison of the efficacy of betaxolol-brinzolamide and timolol-dorzolanmide as suppressors of aqueous humor flow in human subjects. Ophthalmol., 107, 2000: 283–287.

3. Clineschmidt,C.M., Williams, R.D., Snyder, E. et al.: A randomized trial in patients inadequately controlled with timolol alone comparing the dorzolamide-timolol combination to monotherapy with timolol or dorzolamide. Ophthalmol., 105, 1998: 1952–1959.

4. Clineschmidt, C.M., Williams, R.D., Snyder, E. et al.: A randomized trial in patients inadequately controlled with timolol alone comparing the dorzolamide-timolol combination to monotherapy with timolol or dorzolamide. Ophthalmol., 106, 1999, 12 Suppl.: 17–24.

5. Emmerich, K.H.: Comparison of latanoprost monotherapy to dorzolamide combined with timolol in patients with glaucoma and ocular hypertension. A 3-month randomized study. Graefeęs Arch. Clin. Exp. Ophthalmol., 238, 2000: 19–23.

6. Harris, A., Jonescu-Cuypers, C.P., Kagemann, L. et al.:Effect of dorzolamide timolol combination versus timolol 0.5% on ocular blood-flow in patients with primary open-angle glaucoma. Am. J. Ophthalmol., 132, 2001: 490–495.

7. Chiou, G.Y.C., Liu, S.X.L., Li, B.H.P., et al.: Ocular hypotensive effect of L- arginine and its derivates and their actions on ocular blood flow. J. Ocul. Pharmacol. Therapeut., 11, 1995: 1–10.

8. Chiselita, D., Apatachioae, I., Poiata, I.:The ocular hypotensive effect of the combination of latanoprost with dorzolamide. Ophthalmologia, 46, 1999: 39–45.

9. Choudri, S., Wand, M., Shields, M.B.: A comparison of dorzolamide-timolol combination versus the concomitant drugs. Am. J. Ophthalmol., 130, 2000: 832–833.

10. Chuman,, J., Chuman T., Nao-i. N. et al.: The effect of L-arginine on intraocular pressure in the human eye. Current Eye Res., 20, 2000: 511–516.

11. Klimal Arici, M., Topalkara, A., Guler, C.: Additive effect of latanoprost and dorzolamide in patients with elevated intraocular pressure. Int. Ophthalmol., 212, 1998: 37–42.

12. Konstas,A.G., Maltezos,A., Bufidis, T. et al.: Twenty –four hour control of intraocular pressure with dorzolamide and timolol maleate in exfoliation and primary open-angle glaucoma. Eye 14, 2000:73 – 77.

13. Kotikoski, H., Alajuuma, P., Moilanen, E. et al.: Comparison of nitric oxid donor sin lowering intraocular pressure in rabbits:role of cyclic GMP. J. Ocul. Pharmacol. Ther., 18, 2002: 11–13.

14. Liu, S.X., Chiou, G.C., Varma, R.S.:Improvemet of retinal functions after ischemia with L-arginine and its derivates. J. Ocul. Pharmacol. Ther., 11, 1995: 261–265.

15. Martenbaum, D.: The efficacy of dorzolamide, a topical carbonic anhydrase inhibitor, in combination with timolol in the treatment of patients with open-angle glaucoma and ocular hypertension. Clin. Ther., 18,1996:460-465.

16. Maruyama, K., Shirato, S.:Additive effect of dorzolamide or carteolol to latanoprost in primary open-angle glaucoma a prospective randiomized crossover trial. J. Glaucoma 15, 2006: 341–345.

17. Oláh, Z., Veselovský, J., Gressnerová S. et al.: Vnútroočný tlak králikov po instilácii zmesi antiglaukomatika COSOPTu s amínokyselinou L-arginínom.HCl do spojovkového vaku. Čes. a slov. Oftal., 61, 2005:3 – 12.

18. Oláh, Z., Veselovský, J.: Rabbit,s intraocular pressure after instilation of timolol and aminoacid lysine, arginine, glycine or taurine mixture. Bratisl. Med. J., 108, 2007: 283–286.

19. Ormrod, F., McClellan,K.:Topical dorzolamide 2% timolol 0.5%: A review of its use in the treatment of open-angle glaucoma. Drugs Aging., 17, 2000: 477–496.

20. Strohmaier, K., Snyder, E., DuBiner, H. et al.: The efficacy and safety of the dorzolamide-timolol combination versus the concomitant administration of its components. Ophthalmol., 105, 1998:1936 – 1944.

21. Strohmaier, K., Snyder, R., DuBiner,H. et al.:The efficacy and safety of the dorzolamide-timolol combination versus the concomitant administration of its components. Opthalmol., 106, 1999, 12 Suppl.:1 – 9.

22. Susanna, R.Jr., Nicolela, M.T., Oga, E.: Additive effect of latanoprost of the combination of timolol and dorzolamide. J. Glaucoma, 9, 2000: 183–186.

23. Vanlandigham, B.D., Brubaker, R.F.: Combined effect pof dorzolamide and latanoprost on the rate of aqueous humor flow. Amer. J. Ophthalmol., 126, 1998: 191–196.

24. Veselovský, J., Oláh, Z., Veselá, A. et al.: Zmes timptolu s L-arginom.HCl: regulátor vnútroočného tlaku u králikov. Čes. a slov. Oftal., 59, 2003:295–302.

25. Veselovský J., Oláh, Z., Veselá, A. et al.: Reakcia fyziologického vnútroočného tlaku králika na Latanoprost (Xalatan) a jeho zmesi s amínokyselinou L-arginínom.HCl. Čes. a slov. Oftal., 60, 2004:319 – 327.

26. Veselovský, J., Oláh, Z., Veselá, A. et al.: Účinok glycínu s Timoptolom na fyziologické hladiny VOT králikov. Čes. a slov. Oftal., 62, 2006:2 47–254.

27. Wayman, L.L., Larson, L.I., Maus, T.L., et al.: Additive effect of dorzolamide on aqueous humor flow in patients receiving long-term treatment with timolol. Arch. Ophthalm., 116, 1998: 1348–1440.

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