Immunofluorescent Analysis of Anterior Lens Capsule Allotransplantation forChronic Corneal Ulcers

Czech version

Authors: I. Kozák ¹; A. Trbolová ²; J. Rosocha ³; J. Jautová ⁴; T. Juhás ¹; V. Ledecký
Authors‘ workplace: Očná klinika LF UPJŠ, Košice, prednosta doc. MUDr. T. Juhás, DrSc. Klinika chirurgie, ortopédie a roentgenológie, Univerzita Veterinárskeho lekárstva, Košice, prednosta doc. MUDr. V. Ledecký, CSc. Združená Tkanivová banka LF UPJŠ, Košice, prednosta MVDr.
Published in: Čes. a slov. Oftal., , 2004, No. 4, p. 261-266
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Overview

This study uses immunofluorescent analysis to find out in histologic cornealsections a possible rejection reaction in anterior lens capsule allotransplantationfor chronic corneal ulcers. The experiment included control group of rabbitcorneas with repeat injury to epithelium and anterior stroma as well as surgicallytreated group. Fluorescein-conjugated human antibodies to IgA, IgM, IgG, complementand fibrinogen were used in the study. For detection we used method ofdirect immunofluorescence. Positive was only examination on fibrinogen in sectionsof treated group eyes 2 days after surgery. Last examination after 3 monthsfailed to detect positivity in any of the tested markers.We preliminarily concludethat anterior lens capsule allotransplantation for chronic corneal ulcers does notresult in rejection reaction and that components of humoral immunity do notparticipate in immune reaction.

Key words:
anterior lens capsule, allotransplantation, corneal ulcer, immunofluorescentanalysis

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