Quantitative Mass Spectrometry and Its Utilization in Oncology

Czech version

Authors: L. Hernychová; P. Dvořáková; E. Michalová; B. Vojtěšek
Authors‘ workplace: Regionální centrum aplikované molekulární onkologie, Masarykův onkologický ústav, Brno
Published in: Klin Onkol 2014; 27(Supplementum): 98-103

Overview

Cancers are genetically and clinically very heterogeneous diseases; therefore, various proteomic studies have been trying to find biomarkers which can facilitate prognosis, diagnosis or treatment of these oncological diseases. The mass spectrometry is an effective tool for identification, quantitation, and characterization of biomolecules in the complex biological samples. The first step suitable for selection of biomarkers called discovery proteomics provides a detailed analysis of the samples contributing to the identification of proteins, comparison of their presence in the samples, and selection of the convenient candidates for the prospective biomarkers. The next step of proteomics analysis is directed towards verification of chosen biomarkers with the approach called targeted proteomics. This technique evaluates presence and quantity of the proteins (biomarkers) in clinically precisely defined samples. This article focuses on the description of various approaches suitable for the quantitative analysis of the proteins connected with mass spectrometry.

Key words:
quantitative proteomics –⁠ mass spectrometry –⁠ protein –⁠ biomarker –⁠ oncology –⁠ cancer

This work was supported by the European Regional Development Fund and the State Budget of the Czech Republic (RECAMO, CZ.1.05/2.1.00/03.0101) and by MH CZ –⁠ DRO (MMCI, 00209805).

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.

Submitted:
20. 1. 2014

Accepted:
7. 4. 2014

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