Authors: I. Kozák 1; J. Rosocha 2; H. Hrbková 2; T. Juhás 1 Authors‘ workplace: Očná klinika LF UPJŠ, Košice, prednosta doc. MUDr. T. Juhás, DrSc. 2Združená Tkanivová Banka FNsP a LF UPJŠ, Košice, prednosta MVDr. J. Rosocha, CSc. 1 Published in: Čes. a slov. Oftal., , 2003, No. 3, p. 171-175 Category:
The purpose of this studywas to identify limbal stemcells in the culture of humandonor cornea. After standard cultivation procedure we usedmonoclonal antibodyagainst alpha-enolase for identification of stem cells. This antibody serves asa probe for the identification of biochemical marker of stem cells. Using immunofluorescentmicroscopy we identified in specimens -enolase positive cellswhichin vitro but not in situshowed alsosomemorphologic differencesfrom othercellular elements of the culture.
Key words: limbal stem cells, alpha-enolase, immunofluorescent microscopy
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