Metallic nanoparticles affected by therapeutic ultrasound – The in vitro study of cell viability
Authors:
Vladan Bernard 1; Adéla Kalužová 1,2
Authors‘ workplace:
Masarykova univerzita, Lékařská fakulta, Biofyzikální ústav, Brno, Česká republika
1; Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Ústav biomedicínského inženýrství, Brno, Česká republika
2
Published in:
Lékař a technika - Clinician and Technology No. 1, 2014, 44, 5-8
Category:
Original research
Overview
Nanotechnology and nanomaterials increasingly intervene in the life of an ordinary person. These belong to the technology and materials with the promise of high versatility. But, their action and effects on living organisms are still not fully understood. For this reason, nanotechnology exhibits an extreme surge of interest in the field of research of numerous scientific institutions. The objects of interest of the presented in vitro research are metallic nanoparticles and their effect on human tumor cell lines. We studied viability of A375 tumor cell lines during their cultivation in an environment of silver nanoparticles <100 nm and current application of ultrasonic field with intensity of 1 W·cm-2. The outcomes from experiment show decreasing of viability of human tumor cell after exposing of ultrasound and nanoparticles separately, the maximal decreasing of cell viability was documented for combined treatment when ultrasound and nanoparticles were applied at the same time.
Keywords:
Cells, nanoparticles, nanomaterials, viability
Sources
[1] Emerich, D. F., Thanos, C. G., Nanotechnology and medicine. Expert Opin Biol Ther 2003, 3, 655-663.
[2] Sahoo, S. K., Parveen, S., Panda, J. J., The present and future of nanotechnology in human health care, Nanomedicine, Nanotechnology, Biology, and Medicine 2007, 3, 20-31.
[3] Ahamed, M., AlSalhi, M. S:, Siddiqui, M. K. J., Silver nanoparticle applications and human health Clinica Chimica Acta, Volume 2010, vol. 411, p. 1841-1848.
[4] Woodrow Wilson International Center for Scholars. A nanotechnology consumer products inventory. www.nanotechproject.org/consumerproducts 2007 Available at.
[5] Bao Yong Sha, Wei Gao, Shu Qi Wang, Feng Xu, Tian Jian Lu Cytotoxicity of titanium dioxide nanoparticles differs in four liver cells from human and rat Original Research Article Composites Part B: Engineering, Volume 42, Issue 8, December 2011, Pages 2136-2144.
[6] Lanone, S., Rogerieux, F., Geys, J., Dupont, A., Maillot-Marechal, E., Boczkowski, J., Lacroix, G., Hoet, P., Comparative toxicity of 24 manufactured nanoparticles in human alveolar epithelial and macrophage cell lines, Particle and Fibre Toxicology 2009, 6:14.
[7] Ahamed, M., AlSalhi. M. S., Siddiqui. M. K. J., Silver nanoparticle applications and human health, Clinica Chimica Acta 2010, 441, 1841-1848.
[8] Mukherjee, S. G., O Ćlaonadh, N., Casey, A., Chambers, G. Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines, Toxicology in Vitro 2012, 26, 238-251.
[9] Lanone, S., Rogerieux, F., Geys, J., Dupont, A., Maillot-Marechal, E., Boczkowski, J., Lacroix, G., Hoet, P., Comparative toxicity of 24 manufactured nanoparticles in human alveolar epithelial and macrophage cell lines, Particle and Fibre Toxicology 2009, 6, 1-12.
[10] Sha, B., Gao, W., Wang S., Xu, F., Lu, T., Cytotoxicity of titanium dioxide nanoparticles differs in four liver cells from human and rat, Composites: Part B 2011, 42, 2136-2144.
[11] Ionel Rosenthal, I., Sostaric, J. Z., Riesz, P., Sonodynamic therapy-a review of the synergistic effects of drugs and ultrasound Ultrasonics Sonochemistry 2004, 11, 349–363.
[12] Junru Wu, J., Pepe, J., Rincón, M., Sonoporation, anti-cancer drug and antibody delivery using ultrasound, Ultrasonics 2006, 44, e21-25.
[13] Mehier-Humbert, S., Bettinger, T., Yan, F., Guy, R. H., Plasma membrane poration induced by ultrasound exposure: Implication for drug delivery, Journal of Controled Release 2005, 104, 213-222.
[14] Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 1983, 16, 55-63.
Labels
BiomedicineArticle was published in
The Clinician and Technology Journal
2014 Issue 1
Most read in this issue
- A method of compliance measurement and gastight testing in models of the respiratory system
- Metallic nanoparticles affected by therapeutic ultrasound – The in vitro study of cell viability
- Rational operation of MRI equipment in university hospitals in the Czech Republic
- Individualization of head related transfer function