Effect of concentration on transdermal absorption of pyrene in vitro

Czech version

Authors: L. Kotingová ¹; V. Voříšek ²; E. Čermáková ³; L. Borská ⁴; Z. Fiala ¹
Authors‘ workplace: Ústav hygieny a preventivního lékařství, Univerzita Karlova v Praze, Lékařská fakulta v Hradci Králové, přednostka doc. MUDr. Jindra Šmejkalová, CSc. ¹; Ústav klinické biochemie a diagnostiky, Fakultní nemocnice Hradec Králové, přednosta prof. MUDr. Vladimír Palička, CSc. ²; Oddělení výpočetní techniky, Univerzita Karlova v Praze, Lékařská fakulta v Hradci Králové, vedením pověřený Ing. Jiří Špulák ³; Ústav patologické fyziologie, Univerzita Karlova v Praze, Lékařská fakulta v Hradci Králové, přednosta prof. MUDr. Miroslav Kuba, DrSc. ⁴
Published in: Pracov. Lék., 64, 2012, No. 1, s. 18-22.
Category: Original Papers

Overview

The objective of the experiment was to determine the effect of pyrene concentration on transdermal absorption in vitro. A static 10 ml diffusion chamber was used for the testing with absorption area of 1.77 cm². The skin of the pig posterior side of auricle formed the penetration membrane. Three donor concentrations of pyrene (0.00095 and 0.0095 and 0.095 g pyrene/g acetone) were tested. The receptor fluid was composed from the Hank’s solution, bovine serum albumin and gentamicin and was sampled in the intervals of 12, 24, 48 and 72h. The samples were analyzed by means of gas capillary chromatography connected with mass spectrometer. The pyrene concentration in the receptor fluid increased with the time of exposure, but differences between individual concentrations used were never statistically significant. The lag time values were the only significant differences observed (p = 0.047; α = 0.05).

Key words:
skin – in vitro absorption – pyrene – polycyclic aromatic hydrocarbons

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