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Paternity Testing Based on the Analysis of DNA and his Revision


Authors: R. Vrtěl 1,2;  R. Vodička 1;  S. Loyka 2;  J. Šantavý 1
Authors‘ workplace: Ústav lékařské genetiky a fetální medicíny, FN a LF UP, Olomouc 1;  Ústav soudního lékařství a medicínského práva, FN a LF UP, Olomouc 2
Published in: Soud Lék., 53, 2008, No. 1, p. 8-10

Overview

Paternity testing is nowadays mostly based on the analysis of DNA short tandem repeats (STR). Number and selection of STR loci differ according to identification kit producers and also particular laboratories. This article refers a revision of formerly excluded paternity. The cause of the mismatch was revealed by the enlargement of STR loci panel and reciprocal evaluation of samples and paternity was on the contrary confirmed.

Different possibilities of failures, their consequences and preventions are also discussed.

Key words:
paternity – DNA – short tandem repeats – STR – revision


Sources

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2. Edwards, A., Hammond, H.A., Jin, L., Caskey, C.T., Chakraborty, R.: Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics. 1992 Feb;12(2):241-53.

3. Hammond, H.A., Jin, L., Zhong, Y., Caskey, C.T., Chakraborty, R.: Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet. 1994 Jul;55(1):175-189.

4. Santos, S.M., Budowle, B., Smerick, J.B., Keys, K.M., Moretti, T.R.: Portuguese population data on the six short tandem repeat loci—CSF1PO, TPOX, THO1, D3S1358, VWA and FGA. Forensic Sci Int. 1996 Dec 27;83(3):229-35.

5. Sparkes, R., Kimpton, C., Watson, S., Oldroyd, N., Clayton, T., Barnett, L., Arnold, J., Thompson, C., Hale, R., Chapman, J., Urquhart, A., Gill, P.: The validation of a 7-locus multiplex STR test for use in forensic casework. (I). Mixtures, ageing, degradation and species studies. Int J Legal Med. 1996;109(4):186-94.

6. Technical manual of Power Plex TM 16 System.Promega Corporation, Madison (2001)

7. Hummel, K.: Principles of serostatistic paternity evaluation Anthropol Anz. 1976 Mar;35 (2-3):106-113.

8. German.American Association of Blood Banks http://www.cstl.nist. gov/biotech/strbase/mutation.htm

Labels
Anatomical pathology Forensic medical examiner Toxicology
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