Kissová R., Maďarová L., Klement C.: Laboratory Diagnosis of Pandemic Influenza at the Department of Medical Microbiology of the Regional Authority of Public Health Based in Banská Bystrica in the Season 2009–2010

Czech version

Authors: R. Kissová; L. Maďarová; C. Klement
Authors‘ workplace: Regionálny úrad verejného zdravotníctva so sídlom v Banskej Bystrici, Odbor lekárskej mikrobiológie
Published in: Epidemiol. Mikrobiol. Imunol. 60, 2011, č. 1, s. 32-37

Overview

The Department of Medical Microbiology of the Regional Authority of Public Health (RAPH) in Banská Bystrica serves as a catchment laboratory of virology for the Central Slovakia Region, and in the influenza season 2009/10, it also served as such for the East Slovakia Region.

Material and methods:
Specimens (nasopharyngeal swabs and post-mortem specimens) from patients with suspected influenza were obtained from both sentinel and non-sentinel physicians. The specimens were analyzed by a rapid test, followed by real-time PCR (RT-PCR) for influenza A or B diagnosis. RT-PCR subtyping for pandemic influenza A/H1N1 was performed.

Results:
From May 2009 to June 2010, 2497 specimens were analyzed for the presence of influenza A and B viruses and in particular for the presence of pandemic influenza A/H1N1 virus. As many as 537 of 589 influenza A-positive specimens, i.e. 21.5% of all specimens analyzed and 91.2% of influenza A-positive specimens, were subtyped as pandemic influenza A/H1N1.

Conclusion:
In the influenza season 2009/10, the new pandemic influenza A/H1N1 clearly predominated in Central and Eastern Slovakia. PCR tests have played a key role in diagnosing patients with suspected pandemic influenza in the laboratory participating in the surveillance of influenza and influenza-like illness in the Slovak Republic.

Keywords:
pandemic influenza A/H1N1 – respiratory viruses – PCR diagnosis – influenza surveillance.

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Article was published in

Epidemiology, Microbiology, Immunology

2011 Issue 1