Comparison of Some Extraction Methods for the Detection of EhrlichianDNA in Full Blood by Means of PCR

Czech version

Authors: P. Zeman
Authors‘ workplace: KHS Středočeského kraje, Praha
Published in: Epidemiol. Mikrobiol. Imunol. , 2002, č. 4, s. 143-147
Category:

Overview

The author compared nine methods for isolation of specific DNA of the etiological agent of humangranulocytic ehrlichiosis from human blood for examination of the PCR. To full blood of healthydonors a laboratory culture of the agent was added and the effectiveness of isolation an stability ofthe template was tested under conditions when the blood was fresh or frozen. For universal useQIAamp® (QIAGEN) was most suitable, frozen blood was extracted best using NucleoSpin® Tissue(Macherey-Nagel).

Key words:
human granulocytic ehrlichiosis (HGE) –⁠ Anaplasma phagocytophila –⁠ polymerase chainreaction (PCR) –⁠ DNA extraction.

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Article was published in

Epidemiology, Microbiology, Immunology

2002 Issue 4