Authors: D. Chládek 1; J. Pěknicová 1; J. Čapková 1; G. Geussová 1; Olga Teplá 2; J. Madar 3 Authors‘ workplace: Ústav molekulární genetiky AV ČR, Praha - Krč, ředitel prof. MUDr. J. Svoboda, DrSc. 2 ISCARE IVF, a. s., Praha - Hloubětín, ředitel M. Kadlec 3 Ústav pro péči o matku a dítě, Praha - Podolí, ředitel ing. V. Wollmuth 1 Published in: Ceska Gynekol 2000; (1): 28-32 Category:
Objective: Use of monoclonal antibodies against sperm proteins in human medicine.Design: Experimental and clinical studies.Setting: 1 Dep. Biology and Biochemistry of Fertilization, Institute of Molecular Genetics, Prague,2 Laboratory IVF, Iscare IVF, Prague, 3 Dep. of Immunobiology, Institute for the Care of Motherand Child, Prague.Methods: Monoclonal antibodies against human sperm intra-acrosomal and cell surface proteinswere used for quantitative analysis of these proteins by the immunofluorescence test in samplesof human sperm of good and poor qualities.Results: The detection of intra-acrosomal proteins was decreased and, on the other hand, detecti-on of surface proteins was the same or higher in pathological spermatozoa.Conclusion: Monoclonal antibodies can be used for diagnostics of sperm pathology (quantitativedetection of proteins) and for evaluation of the physiological state of sperm cells (state of acroso-me before or after acrosome reaction). Finally, monoclonal antibodies could be useful for selecti-on of a suitable method of fertilization (IUI, standard IVF, ICSI) in the laboratories of assistedreproduction.
Key words: monoclonal antibodies, infertility, sperm pathology, assisted reproduction, IVF met-hods
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