Authors: F. Bilka; M. Vanko; A. Balažová; A. Bílková; I. Holková Authors‘ workplace: Univerzita Komenského v Bratislave, Farmaceutická fakulta, Katedra bunkovej a molekulárnej biológie liečiv Published in: Čes. slov. Farm., 2007; 56, 90-94 Category: Original Articles
Greater celandine, similarly as other plants of the family Papaveraceae, produces benzylisoquinoline alkaloids, primarily benzophenanthridines. Polyphenoloxidase (PPO) is most probably involved in the formation of dopamine, which is one of the precursors of norcoclaurine, the first intermediate with the benzylisoquinoline structure. This study has revealed that PPO present in the latex of greater celandine is localized in the organelles, which serve to store alkaloids (the so-called 1000 g organelles). The enzyme was purified by means of affinity chromatography into electrophoretic homogeneity. It possesses a relative molecular mass of approximately 65 kDa and exerts two activities, the monophenolase and diphenolase ones. With the use of a polymerase chain reaction, it was possible to amplify a part of the PPO gene from the region of the active site.
Key words: Chelidonium majus L. – polyphenoloxidase – latex – sanguinarine
Don‘t have an account? Create new account
Enter the email address that you registered with. We will send you instructions on how to set a new password.
