Morin in the Therapy of the Ischemia-Reperfusion Damage Model of the Rat Kidney


Authors: L. Bartošíková ;  J. Nečas ;  V. Suchý 1;  D. Jankovská 1;  E. Janoštíková ;  T. Bartošík 2;  J. Klusáková 3;  J. Juřica 4;  T. Florian ;  M. Frydrych ;  J. Krčmář ;  P. Fráňa 5;  J. Fráňová 6
Authors‘ workplace: Veterinární a farmaceutická univerzita Brno, Farmaceutická fakulta, Ústav humánní farmakologie a toxikologie ;  Veterinární a farmaceutická univerzita Brno, Farmaceutická fakulta, Ústav přírodních léčiv 1;  Úrazová nemocnice, Brno 2;  Lékařská fakulta Masarykovy univerzity, I. Patologicko-anatomický ústav, Brno 3;  Lékařská fakulta Masarykovy univerzity, Farmakologický ústav, Brno 4;  Fakultní nemocnice U sv. Anny, II. interní klinika, Brno 5;  Dětská nemocnice FN, Brno 6
Published in: Čes. slov. Farm., 2006; 55, 78-83
Category: Original Articles

Overview

The aim of this study was to analyze the protective effects of morin administered during the therapy of reperfusion injury of the laboratory rat kidney. Animals were randomly divided into five groups (n=10). One group was left intact. Three medicated groups and one placebo group were subjected to ischemia (60 min) and reperfusion of the left kidney. Morin was suspended in a 2 ml of 0.5% Avicel solution and administered orally by a gastric probe at doses of 5, 10, and 20 mg.kg⁻¹ once a day for 15 days. The placebo group was given only 2 ml of 0.5% Avicel in the same way. On the 15th day, all the animals were exsanguinated and the reperfused kidneys were recovered. Selected biochemical markers in blood were assessed: superoxide dismutase, glutathion peroxidase, total antioxidative capacity, malondialdehyde, creatinine, urea, and uric acid. Creatinine, urea, and total protein were analyzed in urine, and a 24-hour diuresis was recorded. The kidney tissue samples were used for histopathological examination. Morin supported the organism’s own defensive reactions against free radicals and decreased lipid peroxidation in the cell membranes and contributed to the recovery of kidney functions. The histopathological results confirm 20 mg.kg⁻¹ as the most effective dose.

Key words s:
antioxidative effect – ischemia-reperfusion – kidney – morin – therapy


Labels
Pharmacy Clinical pharmacology
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