Authors: A. Fedurcová 1; J. Lehotay 1; T. Liptaj 1; N. Prónayová 1; J. Čižmárik 2 Authors‘ workplace: Slovenská technická univerzita v Bratislave, Fakulta chemickej a potravinárskej technológie, Ústav analytickej chémie, SR 1; Farmaceutická fakulta Univerzity Komenského v Bratislave, Katedra farmaceutickej chémie, SR 2 Published in: Čes. slov. Farm., 2006; 55, 124-130 Category: Original Articles
Chiral ß-cyclodextrin was used to separate diazepam conformers. Several mobile phases of the composition acetonitrile/acetate buffer 200 mmol/l (pH=3.3, 5.5, 6.5) were employed for this purpose. As follows, the influence of addition of chiral ß-cyclodextrin to the mobile phase on diazepam separation was studied. The interconversion was a concurrence process of separation, resulting from stereolability of the diazepam molecule. The influences of temperature, flow rate, pH, and ionic strength of the mobile phase on interconversion and chromatographic parameters (retention factor and selectivity coefficient) were studied. Complementary off-line NMR measurements were carried out with the goal to confirm the structure of diazepam in the presence of an acid mobile phase.
Key words: interconversion and separation – diazepam – HPLC – NMR
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