Authors: T. Hudecová; Š. Hatrík; N. Zimová; Emil Havránek Authors‘ workplace: Katedra farmaceutickej analýzy a nukleárnej farmácie Farmaceutickej fakulty Univerzity Komenského, Bratislava Published in: Čes. slov. Farm., 2002; , 91-95 Category:
The present paper introduces a rapid HPLC method for the determination of dioxopromethazine and phenylephrine in eye drops. The method uses a modified C18 stationary phase optimized for the separation of basic compounds and a methanoUl.5 mM phosphoric acid (60/40 v/v, pH 3.02) mobile phase. The flow rate is set to 2 mUmin, sample volume 20 pl, and compounds are detected at 275 nm. Prior to analysis, the eye drops are diluted with water in a rado of 1 : 50. The elaborated HPLC method and the chromatographic systém esere validated according to the procedure for the validation of chromatographic systems and methds.
Key words: HPLC/UC - dioxopromethazine - phenylephrine - eye drops - validation
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