Immunohistochemical Characterization of Collagen IV in Control Corneas and in Corneas Obtained from Patients Suffering from Posterior Polymorphous Corneal Dystrophy
Authors:
S. Merjavá 1; P. Lišková 1; K. Jirsová 1,2
Authors‘ workplace:
Laboratoř biologie a patologie oka, Institut dědičných metabolických chorob, VFN a 1. LF UK, Praha, vedoucí pracoviště prof. MUDr. Milan Elleder, DrSc.
1; Oční tkáňová banka, VFN a UK, Praha, vedoucí pracoviště MUDr. Marcela Černická, MBA
2
Published in:
Čes. a slov. Oftal., 64, 2008, No. 3, p. 115-119
Overview
The expression of all six chains of collagen IV was studied using the indirect fluorescent immunohistochemistry in seven control corneas and seven corneas obtained from patients suffering from the posterior polymorphous corneal dystrophy. Heterogeneous staining, especially in the epithelial basement membrane and Descemet’s membrane, was observed in the control corneas. An increase of the staining intensity for the α1 and α2 chains was observed, especially in the Descemet’s membrane and the corneal stroma in samples obtained from the patients compared to the control tissues.
Key words:
collagen IV, cornea, indirect fluorescent immunohistochemistry
Sources
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OphthalmologyArticle was published in
Czech and Slovak Ophthalmology
2008 Issue 3
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