Corynebacterium durum and possibilities of its identification

Czech version

Authors: P. Vítková; O. Bechyňková; J. Scharfen; V. Buchta
Authors‘ workplace: Oddělení lékařské mikrobiologie a imunologie, Oblastní nemocnice Trutnov, a. s. ¹; Lékařská fakulta v Hradci Králové, Univerzita Karlova, Ústav klinické mikrobiologie, Fakultní nemocnice Hradec Králové ²
Published in: Epidemiol. Mikrobiol. Imunol. 74, 2025, č. 2, s. 113-117
Category:
doi: https://doi.org/10.61568/emi/11-6492/20250428/140417

Overview

Corynebaterium (C.) durum is a part of the resident human oral microbiota. Its role in the aetiology of infectious diseases is ambiguous. With the increasing number of immunocompromised patients, it must be considered a potential opportunistic pathogen. It is isolated from the sputum, bronchoalveolar-lavage fluid, as well as blood, especially from immunocompromised patients with pneumonia. In that case, the critical steps involve a correct identification of Corynebacterium to the species level and right interpretation of the findings. The previously widely used commercial test for the identification of Corynebacteria (API Coryne, BioMerieux) is not suitable for all species, including C. durum, as its biotype number is not included in the database. But the obtained result can be compared with the available literature data. Chemotaxonomic and proteomic analysis (matrix-assisted laser desorption/ ionization – time of flight, MALDI-TOF MS) or 16S rRNA sequencing allow for accurate differentiation from the other Corynebacteria species. Nevertheless, these methods are not routinely used in clinical laboratories. A polyphasic approach to the taxonomy based on the data from combined laboratory tests is crucial.

Keywords:

identification – Corynebacterium durum – MALDI-TOF MS – API Coryne

Sources

Barrett SL, Cookson BT, Carlson L, et al. Diversity within Reference Strains of Corynebacterium matruchotii Includes Corynebacterium durum and a Novel Organism. Journal of Clinical Microbiology, 2001,39(3):934–938.
Bengali C, Rossi V, Dolina M et al. Matrix Assisted Laser Desorption Ionization – Time of Flight Mass Spectrometry for Identification of Clinically Relevant Bacteria. Public Library of Science, 2011,6(1):e16424.
Bernard KA, Munro C, Wiebe D, et al. Characteristics of Rare or Recently Described Corynebacterium Species Recovered from Human Clinical Material in Canada. Journal of Clinical Microbiology, 2002,40(11):4375-4381.
Bizzini A, Durussel C, Bille J, et al. Performance of Matrix-Asissted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Bacteria Strains Routinely Isolated in a Clinical Microbiology Laboratory. Journal of Clinical Microbiology, 2010, 48(5):1549–1554.
Claridge JE. Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. Clinical Microbiology Reviews, 2004,17(4):840–862.
Coyle MB and Lipsky BA. Coryneform Bacteria in Infectious Diseases: Clinical ans Laboratory Aspects. Clinical Microbiology Reviews, 1990,3(3):2007–246.
Drancourt M, Bollet C, Carlioz A, et al. 16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentificable Bacterial Isolates. Journal of Clinical Microbiology, 2000,38(10):3623–3630.
Eungyung L, Suhyun P, Sunwoo U, et al. Article Microbiome of Saliva and Plaque in Children According to Age and Dental Caries Experience. Diagnostics, 2021,11(8), 1324. Dostupné na www: https://doi.org/10.3390/diagnostics11081324.
Francavilla R, Ercolini D, Piccolo M. Salivary Microbiota and Metabolome Associated with Celiac Disease. Applied and Environmental Microbiology, 2014,80(11): 3416–3425.
Funke G, von Graevenitz A, Clarridge JE, et al. Clinical Microbiology of Coryneform Bacteria. Clinical Microbiology Reviews, 1997,10(1):125–159.
Von Graevenitz A, Pünter-Stret V, Riegel P, et al. Coryneform Bacteria in Throat Coultures of Healthy Individuals. Journal of Clinical Microbiology, 1998,36(7):2087–2088.
Cherkaoui A, Hibbs J, Emonet S et al. Comparasion of Two Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spetrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level. Journal of Clinical Microbiology, 2010,48(4):1169–1175.
Konrad R, Berger A, Hubert I, et al. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry as a Tool for Rapid Diagnosis of Potentialy Toxigenic Corynebacterium Species in the Laboratory Management of Diphteria-Associated Bacteria. Euro Surveillance, 2010,15(43):9–13.
Lee E, Park S, Um S, et al. Microbiome of Saliva and Plaque in Children According to Age and Dental Caries Experience. Diagnostics (Basel), 2021,11(8):1324. DOI: 10.3390/diagnostics11081324.
Qudeimat M, Alyahya A, Karched M, et al. Dental plaque microbiota profiles of children with caries-free and caries-active dentition. J Dent., 2021,104:103539. DOI: 10.1016/j. jdent.2020.103539.
Redanz U, Redanz S, Treerat P, et al. Differential Response of Oral Mucosal and Gingival Cells to Corynebacterium durum, Streptococcus sanguinis, and Porphyromonas gingivalis Multispecies Biofilms. Front Cell Infect Microbiol., 2021,11,686479. DOI: 10.3389/fcimb.2021.686479.
Riegel P, Heller R, Prevost G, et al. Corynebacterium durum sp. Novel., from Human Clinical Specimens. International Journal of Systematic Bacteriology, 1997,47(4):1107–1111.
Risch M, Radjenovic D, Han JN, et al. Comparasion of MALDI TOF with Conventional Identification of Clinically Relevant Bacteria. Swiss Medical Weekly, 2010,140:w13095.

Sachio T, Hiroshi U, Tomohiro N. Current Status of Matrix-Assisted Laser Desorption/Ionization –Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology. Molecules, 2020,25:4775. DOI: 10.3390/molecules25204775.
Scharfen J. Nokardie – smrtící krásky. Živa, 2007,2:50–52.
Scharfen J. Laboratorní diagnostika onemocnění vyvolaná MAFA. In: Scharfen J, jr. (ed.). Mikroaerofilní aktinomycety a aktinomykóza. Nucleus HK, Hradec Králové, 2010:51–91.
Treerat P, Redanz U, Redanz S, et al. Synergism between Corynebacterium and Streptococcus sanguinis reveals new interactions between oral commensals. ISME J., 2020; 14(5):1154–1169. DOI: 10.1038/s41396-020-0598-2.
Tsuzukibashi O, Uchibori S, Shinozaki-Kzwahara N, et al. A selective medium for the isolation of Corynebacterium species in oral cavities. Journal of Microbiological Methods, 2014,104:(67–71).

Poděkování

Výzkum byl podpořen Grantovou agenturou Univerzity Karlovy, SVV UK, projekt LF HK č. 260544.

Do redakce došlo dne 28. 9. 2024.

Adresa pro korespondenci:
Mgr. Petra Vítková
Oddělení lékařské mikrobiologie a imunologie
Oblastní nemocnice Trutnov a.s.
Maxima Gorkého 77
541 01 Trutnov e-mail: vitkova.petra@nemtru.cz

Labels

Hygiene and epidemiology Medical virology Clinical microbiology

How the epidemiology of rotavirus infections is changing with vaccination in the Czech Republic

Results of microbiological surveillance in patients with high-voltage eletrical injuries: A 10-year single center experience

Microbial contamination of air filters of air conditioning system of urban buses

A lethal tick-borne encephalitis from an active endemic area in Slovakia

Blastocystis in the human gastrointestinal tract – commensal or “silent” pathogen?

Article was published in

Epidemiology, Microbiology, Immunology

2025 Issue 2

Download issue PDF