Authors: P. Křížová 1; J. Kalmusová 1; V. Lebedová 1; J. Felsberg 2; R. Haugvicová 2 Authors‘ workplace: Centrum epidemiologie a mikrobiologie, Státní zdravotní ústav, Praha2Mikrobiologický ústav AV ČR, Praha 1 Published in: Epidemiol. Mikrobiol. Imunol. , 2004, č. 2, s. 74-77 Category:
First results of multilocus sequence typing (MLST) of Haemophilus influenzae strains are presented.MLST of 28 H. influenzae strains isolated frompatients with invasive diseases in the Czech Republicis indicative of clonal homogeneity of these strains: 22 out of 26 H. influenzae b strains tested wereof the same sequence type, ST-6. Four strains were of two sequence types newly described in thisstudy: ST-83 (3 strains) and ST-84 (1 strain). Two nontypeable H. influenzae strains were assigned tosequence types other than ST-6: ST-3 and ST-85 newly described in this study. First MLST resultsshow ST-6 to be typical of H. influenzae b isolated from patients with invasive diseases in the CzechRepublic. The sequence types newly described in this study, i.e. ST-83, ST-84 and ST-85, weresubmitted to the worldwide H. influenzae MLST database (http://haemophilus.mlst.net).
Key words: Haemophilus influenzae – multilocus sequence typing – clonal analysis – sequence type.
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